Cell culture and passaging alters gene expression pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts.docVIP

Neumann et al. Arthritis Research Therapy 2010, 12:R83 /content/12/3/R83 RESEARCH ARTICLE Open Access Cell culture and passaging alters gene expression Research article pattern and proliferation rate in rheumatoid arthritis synovial fibroblasts Elena Neumann*1, Birgit Riepl2, Anette Knedla1, Stephanie Lefèvre1, Ingo H Tarner1, Joachim Grifka3, Jurgen Steinmeyer4, Jurgen Sch?lmerich2, Steffen Gay5 and Ulf Müller-Ladner1 Abstract Introduction: Rheumatoid arthritis synovial fibroblasts (RASF) are key players in synovial pathophysiology and are therefore examined extensively in various e