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Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1.docVIP

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Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1.doc

    Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1

    Zhuetal.AMBExpress2012,2:13 /content/2/1/13 ORIGINAL OpenAccess Cloningandoverexpressionofanewchitosanase genefromPenicilliumsp.D-1 Xu-FenZhu*,Hai-QinTan,ChuZhu,LiLiao,Xin-QiZhangandMinWu Abstract Achitosanasegene,csn,wasclonedfromPenicilliumsp.D-1byinversePCR.ThecDNAsequenceanalysisrevealed thatcsnhadnointron.ThededucedCSNproteinconsistsof250aminoacidsincludinga20-aminoacidsignal peptide,andshared83.6%identitywiththefamily75chitosanasefromTalaromycesstipitatus(B8M2R4).The matureproteinwasoverexpressedinEscherichiacoliandpurifiedwiththeaffinitychromatographyofNi2+-NTA. Thenovelrecombinantchi

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