原创力文档Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells.docVIP
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Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells
Flanaganetal.BiologicalProceduresOnline2012,14:7
/content/14/1/7
Biological Procedures
Online
RESEARCH
OpenAccess
CompetitiveDNAtransfectionformulationvia
electroporationforhumanadiposestemcellsand
mesenchymalstemcells
MichaelFlanagan1,JeffreyMGimble2,GangYu2,XueqingXia3,BruceABunnell4andShulinLi3,5*
Abstract
Background:Adiposestemcellshaveastrongpotentialforuseincell-basedtherapy,butthecurrent
nucleofectiontechnique,whichreliesonunknownbuffers,preventstheiruse.
Results:Wedevelopedanoptimalnucleofectionformulationforhumanadiposestemcellsbyusingathree-step
methodthatwehaddevelopedpreviously.T
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