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Construction of a novel Pichia pastoris strain for production of xanthophylls
Araya-Garayetal.AMBExpress2012,2:24
/content/2/1/24
ORIGINAL ARTICLE
OpenAccess
ConstructionofanovelPichiapastorisstrain
forproductionofxanthophylls
JoséMiguelAraya-Garay1,JoséMAgeitos1,JuanAVallejo1,PatriciaVeiga-Crespo1,2,AngelesSánchez-Pérez3
andTomásGVilla1,2*
Abstract
Inthisstudy,weusedtheyeastcarotenogenicproducerPichiapastorisPp-EBILstrain,whichhasbeenmetabolically
engineered,byheterologouslyexpressingβ-carotene-pathwayenzymestoproduceβ-carotene,asavesselfor
recombinantastaxanthinexpression.Forthispurpose,wedesignednewP.pastorisrecombinant-strainsharboring
astaxanthin-encodinggenesfr
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