Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils.docVIP

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Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils.doc

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Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils

Available online /content/9/2/R25 Research article Open Access Vol 9 No 2 Differential expression of RANK, RANK-L, and osteoprotegerin by synovial fluid neutrophils from patients with rheumatoid arthritis and by healthy human blood neutrophils Patrice E Poubelle, Arpita Chakravarti, Maria J Fernandes, Karine Doiron and Andrée- Anne Marceau Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du Centre Hospitalier de lUniversité Laval (CRCHUL), 2705 boulevard Laurier, Ste-Foy, QC G1V 4G2, Canada Corresponding author: Patrice E Poubelle, Patrice.Poubelle@crchul.ulaval.ca Received: 26 Oct 2006 Revisions requested: 4 Jan 2007 Revisions received: 9 Feb 2007 Accepted: 6 Mar 2007 Published: 6 Mar 2007 Arthritis Research Therapy 2007, 9:R25 (doi:10.1186/ar2137) This article is online at: /content/9/2/R25 ? 2007 Poubelle et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Functional links between bone remodeling and the immune system in chronic inflammatory arthritis are mediated, in part, by the ligand of receptor activator of nuclear factor-kappa-B (RANK-L). Because neutrophils play a crucial role in chronic inflammation, the goal of this study was to determine whether proteins of the RANK/RANK-L pathway are expressed by synovial fluid (SF) neutrophils from patients with rheumatoid arthritis (RA) and to characterize this pathway in normal human blood neutrophils. The expression of RANK-L, osteoprotegerin (OPG), RANK, and tumor necrosis factor receptor-associated factor 6 (TRAF6) was determined by polymerase chain reaction, enzyme-linked immunosorbent assay, Western blotting, and mRNAs encoding OPG and RANK. SF neutrophils from RA patients and normal b