Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction.docVIP

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Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction.doc

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Differential gene expression in incompatible interaction between wheat and stripe rust fungus revealed by cDNA-AFLP and comparison to compatible interaction

Wangetal.BMCPlantBiology2010,10:9 /1471-2229/10/9 RESEARCH ARTICLE OpenAccess Differentialgeneexpressioninincompatible interactionbetweenwheatandstriperustfungus revealedbycDNA-AFLPandcomparisonto compatibleinteraction XiaojieWang1,WeiLiu1,XianmingChen2,ChunleiTang1,YanlingDong1,JinbiaoMa1,XuelingHuang1, GuorongWei1,QingmeiHan1,LiliHuang1,ZhenshengKang1* Abstract Background:Striperustofwheat,causedbyPucciniastriiformisf.sp.tritici(Pst),isoneofthemostimportant diseasesofwheatworldwide.Duetospecialfeaturesofhexaploidwheatwithlargeandcomplexgenomeand difficultiesfortransformation,andofPstwithoutsexualreproductionandhardtocultureonmedia,theuseof mostgeneticandmoleculartechniquesinstudyinggenesinvolvedinthewheat-Pstinteractionshasbeenlargely limited.Theobjectiveofthisstudywastoidentifytranscriptionallyregulatedgenesduringanincompatible interactionbetweenwheatandPstusingcDNA-AFLPtechnique Results:Atotalof52,992transcriptderivedfragments(TDFs)weregeneratedwith64primerpairsand2,437(4.6%) ofthemdisplayedalteredexpressionpatternsafterinoculationwith1,787up-regulatedand650down-regulated. Weobtainedreliablesequences(100bp)for255selectedTDFs,ofwhich113(44.3%)hadputativefunctions identified.Alargegroup(17.6%)ofthesegenessharedhighhomologywithgenesinvolvedinmetabolismand photosynthesis;13.8%togeneswithfunctionsrelatedtodiseasedefenseandsignaltransduction;andthoseinthe remaininggroups(12.9%)togenesinvolvedintranscription,transportprocesses,proteinmetabolism,andcell structure,respectively.ThroughcomparingTDFsidentifiedinthepresentstudyforincompatibleinteractionand thoseidentifiedinthepreviousstudyforcompatibleinteractions,161TDFsweresharedbybothinteractions,94 wereexpressedspecificallyintheincompatibleinteraction,ofwhichthespecificityof43selectedtranscriptswere determinedusingquantitativereal-timepolymerasechainreaction(qRT-PCR).Basedontheanalysesofhomology togenesknowntoplayaroleindefense,signaltransductionandproteinmetabolism,20TDFswerechosenand theirexpressionpatternsrevealedb