Electrooxidation and Determination of Dopamine Using a Nafion？-Cobalt Hexacyanoferrate Film Modified Electrode.docVIP

Sensors 2008, 8, 1950-1959 sensors ISSN 1424-8220 ? 2008 by MDPI /sensors Full Research Paper Electrooxidation and Determination of Dopamine Using a Nafion?-Cobalt Hexacyanoferrate Film Modified Electrode Suely S. L. Castro 1, Roger J. Mortimer 2, Marcelo F. de Oliveira 3 and Nelson R. Stradiotto 4,* 1 UERN - FANAT - Departmento de Química, 59600-010 – Mossoró, RN, Brazil 2 Loughborough University - Department of Chemistry, Loughborough, LE11 3TU, UK 3 USP – Faculdade de Filosofia, Ciências e Letras de Ribeir?o Preto- Departamento de Química, 14040-901 – Ribeir?o Preto, SP, Brazil 4 UNESP – Instituto de Química- Departamento de Química Analítica, 14800-900 – Araraquara, SP, Brazil * Author to whom correspondence should be addressed; E-mail: nrstradi@iq.unesp.br Received: 29 May 2007 / Accepted: 17 March 2008 / Published: 19 March 2008 Abstract: The electrocatalysis of dopamine has been studied using a cobalt hexacyanoferrate film (CoHCFe)-modified glassy carbon electrode. Using a rotating disk CoHCFe-modified electrode, the reaction rate constant for dopamine was found to be 3.5 × 105 cm3 mol-1 s-1 at a concentration of 5.0 × 10-5 mol L-1. When a Nafion? film is applied to the CoHCFe-modified electrode surface a high selectivity for the determination of dopamine over ascorbic acid was obtained. The analytical curve for dopamine presented linear dependence over the concentration range from 1.2 × 10-5 to 5.0 × 10-4 mol L-1 with a slope of 23.5 mA mol-1 L and a linear correlation coefficient of 0.999. The detection limit of this method was 8.9 × 10-6 mol L-1 and the relative standard deviation for five measurements of 2.5 × 10-4 mol L-1 dopamine was 0.58%. Keywords: Cobalt hexacyanoferrate; chemically modified electrode; dopamine; voltammetry; rotating disk electrode. 1. Introduction There is an increasing demand for simple and sensible analytical methods for the det