Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro.docVIP

BioMedical Engineering OnLine BioMedCentral Research Open Access Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro Matej Reber?ek1, Cécile Faurie2, Ma?a Kandu?er1, Selma Жorovi?1, Justin Teissié2, Marie-Pierre Rols2 and Damijan Miklav?i?*1 Address: 1University of Ljubljana, Faculty of Electrical Engineering, Tr?a?ka 25, SI-1000 Ljubljana, Slovenia and 2Institut de Pharmacologie et de Biologie Structurale du CNRS UMR 5089, 205, route de Narbonne, 31077 Toulouse cedex, France Email: Matej Reber?ek - matej.rebersek@fe.uni-lj.si; Cécile Faurie - faurie.cecile@; Ma?a Kandu?er - masa.kanduser@fe.uni-lj.si; Selma Жorovi? - selma.corovic@fe.uni-lj.si; Justin Teissié - justin.teissie@ipbs.fr; Marie-Pierre Rols - marie-pierre.rols@ipbs.fr; Damijan Miklav?i?* - damijan.miklavcic@fe.uni-lj.si * Corresponding author Published: 2 July 2007 Received: 26 February 2007 Accepted: 2 July 2007 BioMedical Engineering OnLine 2007, 6:25 doi:10.1186/1475-925X-6-25 This article is available from: /content/6/1/25 ? 2007 Reber?ek et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Gene electrotransfer is a non-viral method used to transfer genes into living cells by means of high-voltage electric pulses. An exposure of a cell to an adequate amplitude and duration of electric pulses leads to a temporary increase of cell membrane permeability. This phenomenon, termed electroporation or electropermeabilization, allows various otherwise non- permeant molecules, including DNA, to cross the membrane and enter the cell. The aim of our research was to develop and test a new system and protocol that would improve gene electrotransfe