Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis.docVIP

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Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis.doc

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Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis

Molecules 2012, 17, 3761-3773; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Enhancement of Palmarumycin C12 and C13 Production in Liquid Culture of the Endophytic Fungus Berkleasmium sp. Dzf12 by Oligosaccharides from Its Host Plant Dioscorea zingiberensis Yan Li 1,2, Tijiang Shan 2, Yan Mou 2, Peiqin Li 2, Jianglin Zhao 2, Wensheng Zhao 2, Youliang Peng 2, Ligang Zhou 2,* and Chunbang Ding 1,* 1 College of Biology and Science, Sichuan Agricultural University, Yaan 625014, China 2 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China * Authors to whom correspondence should be addressed; E-Mails: lgzhou@ (L.Z.); dcb@ (C.D.). Received: 19 January 2012; in revised form: 21 March 2012 / Accepted: 22 March 2012 / Published: 26 March 2012 Abstract: Three crude oligosaccharides were respectively prepared by acid hydrolysis of three polysaccharides, which were water-extracted polysaccharide (WEP), sodium hydroxide-extracted polysaccharide (SEP) and acid-extracted polysaccharide (AEP) from the rhizomes of Dioscorea zingiberensis. Among the three oligosaccharides, the crude oligosaccharide prepared by acid hydrolysis of WEP was found to be the most efficient elicitor to enhance the production of palmarumycins C12 and C13 in liquid culture of endophytic fungus Berkleasmium sp. Dzf12. When OW was applied to the medium at 300 mg/L on day 3 of culture, the maximal yields of palmarumycin C12 (87.96 mg/L) and palmarumycin C13 (422.28 mg/L) were achieved on day 15 of culture, which were 9.83 and 3.24-fold in comparison with those (8.95 and 130.43 mg/L) of control, respectively. The results indicate that addition of the oligosaccharides from the host plant D. zingiberensis should be an effective strategy for enhancing production of palmarumycins C12 and C13 in liquid culture of endophytic fungus Berkleasmium sp. D