Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor.docVIP

Sensors 2010, 10, 5758-5773; doi:10.3390/s100605758 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Enzymatic Determination of Diglyceride Using an Iridium Nano-Particle Based Single Use, Disposable Biosensor Shu-Yi Hsu 1, Brandon Bartling 2, Christina Wang 3, Fuh-Sheng Shieu 1 and Chung-Chiun Liu 2,* 1 Department of Materials Science and Engineering, National Chung Hsing University, Taichung 40227, Taiwan; E-Mails: g9766119@.tw (S.-Y.H.); fsshieu@.tw (F.S.S.) 2 Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; E-Mail: bxb79@ 3 Department of Biological Sciences, College of Arts and Sciences, Cornell University, 147 Goldwin Smith Hall, Ithaca, NY 14853-3201, USA; E-Mail: cpw39@ * Author to whom correspondence should be addressed; E-Mail: cxl9@; Tel.: +1-216-368-2935; Fax: +1-216-368-8738. Received: 26 April 2010; in revised form: 15 May 2010 / Accepted: 28 May 2010 / Published: 8 June 2010 Abstract: A single use, disposable iridium-nano particle contained biosensor had been developed for the determination of diglyceride (DG). In this study hydrogen peroxide, formed through the enzymatic breakdown of DG via lipase, glycerol kinase and glycerol 3-phosphate oxidase, was electrochemically oxidized at an applied potential of +0.5 V versus the Ag/AgCl reference electrode. The oxidation current was then used to quantify the diglyceride concentration. Optimum enzyme concentrations and the surfactant loading used were established for successful sensor response. Good linear performance was observed over a DG concentration range of 0 to 25 μM in phosphate buffer and bovine serum media. Keywords: diglyceride; disposable biosensor; enzyme 1. Introduction Diglyceride (diacylglycerol, DG) is a simple lipid consisting of a glycerol molecule linked through ester bonds to two fatty acids. Its small