Epidemiology of plasmid-mediated quinolone resistance in salmonella enterica serovar typhimurium isolates from food-producing animals in Japan.docVIP
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Epidemiology of plasmid-mediated quinolone resistance in salmonella enterica serovar typhimurium isolates from food-producing animals in Japan
Asaietal.GutPathogens2010,2:17
/content/2/1/17
SHORT REPORT
OpenAccess
Epidemiologyofplasmid-mediatedquinolone
resistanceinsalmonellaentericaserovar
typhimuriumisolatesfromfood-producing
animalsinJapan
TetsuoAsai1*,ChizuruSato2,KaoriMasani2,MasaruUsui1,ManaoOzawa1,TomoeOgino1,HiroshiAoki2,
TakuoSawada2,HidemasaIzumiya3,HaruoWatanabe3
Abstract
Atotalof225isolatesofSalmonellaentericaserovarTyphimuriumfromfood-producinganimalscollectedbetween
2003and2007wereexaminedfortheprevalenceofplasmid-mediatedquinoloneresistance(PMQR)determinants,
namelyqnrA,qnrB,qnrC,qnrD,qnrS,qepAandaac(6’)Ib-cr,inJapan.Twoisolates(0.8%)ofS.TyphimuriumDT104
fromdifferentdairycowsonasinglefarmin2006and2007werefoundtohaveqnrS1onaplasmidofapproxi-
mately9.6-kbp.NoneoftheS.TyphimuriumisolateshadqnrA,qnrB,qnrC,qnrD,qepAandacc(6’)-Ib-cr.Currentlyin
Japan,theprevalenceofthePMQRgenesamongS.Typhimuriumisolatesfromfoodanimalsmayremainlowor
restricted.ThePFGEprofileoftwoS.TyphimuriumDT104isolateswithoutqnrS1onthefarmin2005hadanidenti-
calPFGEprofiletothoseoftwoS.TyphimuriumDT104isolateswithqnrS1.ThePFGEanalysissuggestedthatthe
alreadyexistingS.TyphimuriumDT104onthefarmfortuitouslyacquiredtheqnrS1plasmid.
Findings
InJapan,fluoroquinolonedrugswereapprovedinveter-
Salmonella enterica serovar Typhimurium is prevalent inaryfieldsin1991andarecommonlyusedfortreatment
inmanyanimal species [1-3] including food-producing ofbacterialdiseasessuchasenteritis andpneumoniain
animals thatareconsidered tobereservoirs forhuman food-producing animals [7]. In2001, fluoroquinolone
infection. S.Typhimuriumwasthetop5serovar found resistancewasfoundinS.Choleraesuisfrompigs[8]and
mostfrequentlyincasesofSalmonellafoodborneillness S.Typhimuriumfromcattle[9].Inaddition,afluoroqui-
in Japan between 2006 and 2010 https://hasseidoko. nolone-resistantS.Typhimuriumwasidentifiedinbovine
mhlw.go.jp/Byogentai/Pdf/data48e.pdf. Multidrug-resis- isolatesin2005[6].Themechanismoffluoroquinolone
tantS.Typhimuriumdefinitivephage
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