Erythropoietin improves skeletal muscle microcirculation and tissue bioenergetics in a mouse sepsis model.docVIP

Available online /content/11/3/R58 Research Open Access Vol 11 No 3 Erythropoietin improves skeletal muscle microcirculation and tissue bioenergetics in a mouse sepsis model Raymond Kao1,2, Anargyros Xenocostas2, Tao Rui2, Pei Yu2, Weixiong Huang2, James Rose2 and Claudio M Martin2 1Department of National Defense, Canadian Forces Medical Group, 1745 Alta Vista Drive, Ottawa, Ontario, K1A 0K6, Canada 2London Health Sciences Center, Divisions of Critical Care Hematology; Center for Critical Illness Research; Lawson Health Research Institute; University of Western Ontario, 800 Commissioners Rd. E., London, Ontario, N6A 5W9, Canada Corresponding author: Raymond Kao, rkao3@uwo.ca Received: 30 Jan 2007 Revisions requested: 27 Feb 2007 Revisions received: 17 Apr 2007 Accepted: 18 May 2007 Published: 18 May 2007 Critical Care 2007, 11:R58 (doi:10.1186/cc5920) This article is online at: /content/11/3/R58 ? 2007 Kao et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Introduction The relationship between oxygen delivery and sham group (28.5 versus 36.6 capillaries per millimeter; p 0.001). Treatment of CLP mice with rHuEPO resulted in an immediate and significant increase in perfused capillaries in the CLP group at all time points compared to baseline from 28.5 to 33.6 capillaries per millimeter at 40 minutes; p 0.001. A significant increase in baseline NADH, suggesting tissue hypoxia, was noted in the CLP mice compared to the sham group (48.3 versus 43.9 fluorescence units [FU]; p = 0.03) and improved with rHuEPO from 48.3 to 44.4 FU at 40 minutes (p = 0.02). Six hours after treatment with rHuEPO, CLP mice demonstrated a higher mean perfused capillary density (39.4 ve