5-9星点设计效应面法优化白茅根总酚酸的提取工艺-幻灯片.docVIP

星点设计-效应面法优化白茅根总酚酸提取工艺 (，陈石生，任刚，邵峰，黄慧莲，杨明 (江西中医学院现代中药制剂教育部重点实验室，江西 南昌 330004) [摘要] 目的：优化白茅根总酚酸的提取工艺。方法：采用超声辅助提取法，选取提取试剂浓度、提取时间、提取温度和料液比作单因素试验，在此基础上采用星点设计考察提取试剂浓度、提取时间及提取温度3因素对提取工艺的影响，对结果进行二项式方程拟合，应面法选最佳条件并进行预测分析。结果：得到的最佳提取工艺条件：乙醇浓度为40%，提取时间3min，提取温度为3 ℃，料液比为1:40，总酚酸含量的预测值为2.7102 mg·g-1。验证实验总酚酸含量为2.4625 mg·g-1，与预测值接近，说明模型可靠。结论：该工艺步骤简单，稳定可行，为白茅根进一步的研究提供科学依据。 [关键词] 白茅根；总酚酸；提取工艺；应面法 Optim Extraction of Total Phenolics from the Roots of Imperata cylindrica Based on Central Composite Design and Response Surface Methodology LIU Rong-hua*, CHEN Shi-sheng, REN Gang, SHAO Feng, HUANG Hui-lian, YANG Ming (Key Laboratory of Modern Preparation of TCM, Jiangxi University of TCM, Ministry of Education, Nanchang 330004, China) [ABSTRACT] AIM: To identify the optimal conditions of extraction technology of total phenolics in Rhizoma Imperatae. METHODS: The influencing factors were studied by the single factor experiment, and central composite design and response surface methodology were applied to optimize the extraction process for total phenolics in terms of the content of total phenolics. RESULTS: The optimum extraction conditions were as follows: 40.00% ethanol as extraction solvent at 32.86 ℃ for 37.81 min, and the ratio of liquid to material was 1:40. Under these conditions, the extraction rate was 2.7102 mg·g-1. CONCLUSION: The process is simple, provided high extraction rate, To provide a scientific basis for further research of Rhizoma Imperatae. [KEY WORDS] Imperata cylindrica; Total Phenolics; Extraction Technology; Central Composite Design; Response Surface Methodology 白茅根为禾本科植物白茅Imperata cylindrica Beauv. Var. major(Nees) C. E. Hubb. 的干燥根茎，又名兰根、地筋、白花茅根、茅草根、甜草根等，广泛分布于全国各地，具有凉血止血、清热生津、利尿通淋之功效[1]。白茅根酚酸类成分有Tricin、Jaceidin、Quercetagetin-3,5,6,3’-tetramethyl ether、3,5-Di-O-methyl-kaempferol、3-Hydroxy-4-methoxy benzaldehyde，Impecyloside，Cylindol A、B，graminones A、B，Imperanene，5-hydroxy-2-(2-phenylethyl)chromone、5-hydroxy-2-styrylchromone、5-hydroxy-2-[2-(2-hydroxyphenyl)ethyl