Evaluation of Antioxidant Activities of Aqueous Extracts and Fractionation of Different Parts of Elsholtzia ciliata.docVIP

Molecules 2012, 17, 5430-5441; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Evaluation of Antioxidant Activities of Aqueous Extracts and Fractionation of Different Parts of Elsholtzia ciliata Xiangping Liu 1,2,?, Jia Jia 3,?, Lei Yang 1,*, Fengjian Yang 1, Hongshuang Ge 1, Chunjian Zhao 1, Lin Zhang 1 and Yuangang Zu 1,* 1 State Engineering Laboratory for Bioresource Eco-Utilization, Northeast Forestry University, Harbin 150040, China; E-Mails: lxp110@ (X.L.); yangfj@ (F.Y.); swx05131ghs@126.com (H.G.); zcjsj@163.com (C.Z.); zhanglin6600@ (L.Z.) 2 College of Animal Science Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China 3 Preclinical Medicine Department of Daqing Medical College, Daqing 163319, China; E-Mail: jiajiaplay2006@163.com (J.J.) ? These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: ylnefu@163.com (L.Y.); zygnefu@163.com (Y.Z.); Tel.: +86-451-8219-1387 (Y.Z.); Fax: +86-451-8210-2082 (Y.Z.). Received: 17 April 2012; in revised form: 25 April 2012 / Accepted: 28 April 2012 / Published: 9 May 2012 Abstract: The aim of this study was to investigate the antioxidant and free-radical scavenging activity of extract and fractions from various parts of Elsholtzia ciliata. The inflorescences, leaves, stems and roots of E. ciliata were extracted separately and two phenolic component enrichment methods: ethyl acetate-water liquid-liquid extraction and macroporous resin adsorption-desorption, were adopted in this study. The antioxidant activities of water extracts and fractions of E. ciliata were examined using different assay model systems in vitro. The fraction root E (purified by HPD300 macroporous resin) exhibited the highest total phenolics content (497.2 ± 24.9 mg GAE/g), accompanied with the highest antioxidant activity against various antioxidant sy