Expression of common fragile sites in two Ceboidea species Saimiri boliviensis and Alouatta caraya (Primates Platyrrhini).docVIP

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Expression of common fragile sites in two Ceboidea species Saimiri boliviensis and Alouatta caraya (Primates Platyrrhini).doc

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Expression of common fragile sites in two Ceboidea species Saimiri boliviensis and Alouatta caraya (Primates Platyrrhini)

Genet.Sel.Evol.32(2000)87–97 c INRA,EDPSciences 87 Original article Expression of common fragile sites in two Ceboidea species: Saimiri boliviensis and Alouatta cara a (Primates: Platyrrhini) ArielaFUNDIAa?,Mar′?aGOROSTIAGAb,MartaMUDRYb a DepartmentofGenetics,HematologyResearchInstitute,“MarianoR.Castex”, NationalAcademyofMedicine,PachecodeMelo3081,C.P.1425,BuenosAires, Argentina b DepartmentofBiology(GIBE),FCEyN,BuenosAiresUniversity, BuenosAires,Argentina (Received7September1998;accepted22November1999) Abstract – Fragile sites are points of preferential breakage that may be involved in chromosome rearrangements. Induction of common fragile sites (c-fra) and sponta- neousbreakagewereanalyzedintwoNewWorldMonkeysspecies:Saimiriboliviensis (SBO)andAlouatta caraya(ACA).Spontaneouschromosomeaberrations wereana- lyzedonuntreatedlymphocytecultureswithBr¨ogger’sformula(1977).SBOpresented a low level of spontaneous breakage, while higher frequencies were detected in ACA inwhichbands1q23;2q13and11q19weresigni?cantlya?ected(p0.01).Thepop- ulational distribution of c-fra was analyzed by the Chi2 test in FUdR plus ca?eine treated cultures. A total of 21 c-fra was identi?ed in SBO and 24 in ACA. Fragile sites A1q33, B1p21, B4p14, C3q23 and C5q22 were identi?ed in all analyzed SBO specimens. The most frequent c-fra identi?ed in ACA specimens were 1q23, 1q31, 1q33,2q22,8q14,12q31,13q22,14q15andXq22.FragilesitesA1q31,A1q33,B1q14, B3q13, B4q21 and Xq22 identi?ed in SBO and 1q31, 1q33, 2q22, 4q21, 6q13, 13q22 and Xq22 from ACA were the most conserved sites. A low coincidence between the locationofc-fraandthatofheterochromatinandbreakpointsinvolvedineuchromatic rearrangementsknownforthesegenera,wasestablished. Ceboidea / fragile sites / chromosomal rearrangements / heterochromatin / evolution R′esum′e – Sites communs de fragilit′e chromosomique chez deux esp`eces de Cebo¨?d′es : Saimiri boliviensis et Alouatta cara a (Primates : Platyrrhini). Les sites fragiles sont des r′egio