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Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis.doc

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Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis

Available online /content/7/3/R445 Research article Open Access Vol 7 No 3 Expression of cytokine mRNA and protein in joints and lymphoid organs during the course of rat antigen-induced arthritis Dirk Pohlers1, Angela Siegling2, Eberhard Buchner3, Carsten B Schmidt-Weber4, Ernesta Palombo-Kinne1, Frank Emmrich5, Rolf Br?uer6 and Raimund W Kinne1 1Experimental Rheumatology Unit, Friedrich Schiller University Jena, Jena, Germany 2EUCODIS GmbH, Vienna, Austria 3Pfizer GmbH, Karlsruhe, Germany 4Swiss Institute for Asthma and Allergy Research (SIAF), Davos, Switzerland 5Institute of Clinical Immunology and Transfusion Medicine, University of Leipzig, Leipzig, Germany 6Institute of Pathology, Friedrich Schiller University Jena, Jena, Germany Corresponding author: Raimund W Kinne, Raimund.W.Kinne@rz.uni-jena.de Received: 4 Nov 2004 Revisions requested: 3 Dec 2004 Revisions received: 4 Jan 2005 Accepted: 11 Jan 2005 Published: 17 Feb 2005 Arthritis Research Therapy 2005, 7:R445-R457 (DOI 10.1186/ar1689) ? 2005 Pohlers et al.;licensee BioMed Central Ltd. /content/7/3/R445 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/ 2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is cited. Abstract Cytokine expression was assessed during antigen-induced arthritis (AIA) in synovial membrane (SM), inguinal lymph node (LN), and spleen using competitive RT-PCR and sandwich ELISA. In the SM, early elevations of IL-1β and IL-6 mRNA (by 6 hours; 450- and 200-fold, respectively) correlated with the joint swelling; a 6-fold increase in tumor necrosis factor α (TNFα) was not significant. Not only IL-2 and IFN-γ (which increased 10,000-fold and 200-fold, respectively), but also IL-5 and IL-10, increased acutely (6 hours – day 1; 3-fold and 35-fold, respectively) in the SM. In general, the protein levels in the SM for IL-1