Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors.docVIP

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Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors.doc

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Expression of fatty acid synthesis genes and fatty acid accumulation in haematococcus pluvialis under different stressors

Leietal.BiotechnologyforBiofuels2012,5:18 /content/5/1/18 RESEARCH OpenAccess Expressionoffattyacidsynthesisgenesandfatty acidaccumulationinHaematococcuspluvialis underdifferentstressors AnpingLei1?,HuanChen1?,GuomingShen2,ZhangliHu1,LeiChen3andJiangxinWang3,4* Abstract Background:Biofuelhasbeenthefocusofintensiveglobalresearchoverthepastfewyears.Thedevelopmentof 4th generationbiofuelproduction(algae-to-biofuels)basedonmetabolicengineeringofalgaeisstillinitsinfancy, oneofthemainbarriersisourlackingofunderstandingofmicroalgalgrowth,metabolismandbiofuelproduction. Althoughfattyacid(FA)biosynthesispathwaygeneshavebeenallclonedandbiosynthesispathwaywasbuiltup insomehigherplants,themolecularmechanismforitsregulationinmicroalgaeisfarawayfromelucidation. Results:WeclonedmainkeygenesforFAbiosynthesisinHaematococcuspluvialis,agreenmicroalgaasa potentialbiodieselfeedstock,andinvestigatedthecorrelationsbetweentheirexpressionalternationandFA compositionandcontentdetectedbyGC-MSunderdifferentstresstreatments,suchasnitrogendepletion,salinity, highorlowtemperature.Ourresultsshowedthathightemperature,highsalinity,andnitrogendepletion treatmentsplayedsignificantrolesinpromotingmicroalgalFAsynthesis,whileFAqualitieswerenotchanged much.Correlationanalysisshowedthatacylcarrierprotein(ACP),3-ketoacyl-ACP-synthase(KAS),andacyl-ACP thioesterase(FATA)geneexpressionhadsignificantcorrelationswithmonounsaturatedFA(MUFA)synthesisand polyunsaturatedFA(PUFA)synthesis. Conclusions:WeproposedthatACP,KAS,andFATAinH.pluvialismayplayanimportantroleinFAsynthesisand mayberatelimitinggenes,whichprobablycouldbemodifiedforthefurtherstudyofmetabolicengineeringto improvemicroalgalbiofuelqualityandproduction. Keywords:Biofuel,Geneexpression,Fattyacidsynthesis,Greenmicroalgae Background includes mainly palmitic acid, stearic acid, oleic acid, Withtheeconomicdevelopment,fossilfuelsfromnon- linoleicacidandotherlong-chainfattyacidsandesters renewable resources willeventually runout. According formed by a