Expression of junctional proteins in choroid plexus epithelial cell lines a comparative study.docVIP

Cerebrospinal Fluid Research BioMedCentral Research Open Access Expression of junctional proteins in choroid plexus epithelial cell lines: a comparative study Joanna Szmydynger-Chodobska, Crissey L Pascale, Andrew N Pfeffer, Cassaundra Coulter and Adam Chodobski* Address: Department of Clinical Neurosciences, The Warren Alpert Medical School of Brown University, Providence, RI 02903, USA Email: Joanna Szmydynger-Chodobska - joanna_szmydynger-chodobska@; Crissey L Pascale - Crissey09@Y; Andrew N Pfeffer - anp3d@; Cassaundra Coulter - Casscoul@; Adam Chodobski* - adam_chodobski@ * Corresponding author Published: 27 December 2007 Received: 16 October 2007 Accepted: 27 December 2007 Cerebrospinal Fluid Research 2007, 4:11 doi:10.1186/1743-8454-4-11 This article is available from: /content/4/1/11 ? 2007 Szmydynger-Chodobska et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: There is an increasing interest in using choroid plexus (CP) epithelial cell lines to study the properties of the blood-cerebrospinal fluid barrier (BCSFB). Currently, there are three major CP-derived cell lines available. Z310 and TR-CSFB3, two immortalized cell lines carrying the simian virus 40 large T-antigen gene, were derived from rat CP epithelium, whereas the CPC-2 cell line was derived from human CP carcinoma. Although these cell lines have previously been used in various functional studies, the expression of adherens junction (AJ) and tight junction (TJ) proteins in these epithelial cells has not been systematically studied. Accordingly, in the present study, we sought to characterize the expression of these junctional proteins in these three cell lines. Methods: The cells