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Exploring systemic RNA interference in insects a genome-wide survey for RNAi genes in Tribolium.doc

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Exploring systemic RNA interference in insects a genome-wide survey for RNAi genes in Tribolium

Open Access Re rch 2008TomoyasuetVoal.lumesea9, Issue 1, Article R10 Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium Yoshinori Tomoyasu*?, Sherry C Miller*?, Shuichiro Tomita?, Michael Schoppmeier§, Daniela Grossmann? and Gregor Bucher? Addresses: *Division of Biology, Kansas State University, Manhattan, Kansas 66506, USA. ?K-State Arthropod Genomics Center, Kansas State University, Manhattan, Kansas 66506, USA. ?Insect Genome Research Unit, National Institute of Agrobiological Sciences, 1-2, Owashi, Tsukuba, Ibaraki 305-8634, Japan. §Universitat Erlangen, Institut fur Biologie, Abteilung fur Entwicklungsbiologie, Staudtstr., D-91058 Erlangen, Germany. ?Johann-Friedrich-Blumenbach-Institut für Zoologie und Anthropologie, Georg-August-Universit?t G?ttingen, Abteilung Entwicklungsbiologie, Justus-von-Liebig-Weg, 37077 G?ttingen, Germany. Correspondence: Yoshinori Tomoyasu. Email: tomoyasu@ Published: 17 January 2008 Received: 20 July 2007 Revised: 13 November 2007 Accepted: 17 January 2008 Genome Biology 2008, 9:R10 (doi:10.1186/gb-2008-9-1-r10) The electronic version of this article is the complete one and can be found online at /2008/9/1/R10 ? 2008 Tomoyasu et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. RNAiinpsecTtrgenessibyostleiuminmicTriboliumresemblesRNAi probC.abelegansly uses aindshowingifferent maerobustchanissystemicm. /p RNAi response, but does not have C. elegans-type RNAi mechanisms; Abstract Background: RNA interference (RNAi) is a highly conserved cellular mechanism. In some organisms, such as Caenorhabditis elegans, the RNAi response can be transmitted systemically. Some insects also exhibit a systemic RNAi response. However, Droso