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Experimental infection of dogs with a feline endogenous retrovirus RD-114.doc

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Experimental infection of dogs with a feline endogenous retrovirus RD-114

Narushimaetal.ActaVeterinariaScandinavica2011,53:3 /content/53/1/3 RESEARCH OpenAccess Experimentalinfectionofdogswithafeline endogenousretrovirusRD-114 RieNarushima1*,NoriyukiHoriuchi1,TatsufumiUsui2,TakashiOgawa1,ToshioTakahashi3,TomoakiShimazaki4 Abstract Background:ThefelineendogenousretrovirusRD114iscontainedinthegenomeofcats.Thevirusmay contaminatelivecaninevaccinesbasedonculturedfelinecells.Theinvivoinfectivity,acuteandsubacute pathogenicity,andviralproliferationoftheRD114viruswereevaluatedbyexperimentalinfectionofdogs. Methods:Ninespecificpathogenfreedogsweredividedintothreegroups,witheachgroupconsistingofone femaleandtwomaledogs.Dogsweresubcutaneouslyinoculatedintheneckwitheither1mlRD114stockvirus (groupA),inactivatedRD114virussuspension(groupB),orcellculturemedium(groupC)asanegativecontrol.To assessbloodcellcountsandbiochemicalproperties,bloodsamplesfromeachgroupwerecollected5daysbefore inoculation,justpriortoinoculation,and1,3,7and10dayspost-inoculation. Result:Duringtheexperimentalperiodof51days,noneofthedogsinoculatedwithRD114virusshowedany clinicalsigns,significantincreasesinrectaltemperatureorabnormalbloodbiochemicalcharacteristicsincluding C-reactiveproteinwhencomparedwiththenegativecontrols.Wewerenotabletore-isolatetheRD114virusfrom buffycoatcellsofgroupAdogs.Additionally,wecouldnotdetectRD114provirusinthegenomicDNAisolated fromperipheralbloodleukocytes,lymphnode,spleenandsternalbonemarrowcells. Conclusions:SignsofRD114virusproliferationwerenotfoundaftersubcutaneousinfectionofdogs.Althoughthe potentialriskcausedbyinfectionwithRD114virusindogscouldnotbeassessedinthisstudy,wesuspectthat RD114virushaslittleornovirulenceindogs. Background etiologicalfeaturesofRD114virusinfectionindogs.The Domesticcatsaregenerallyassumedtoharbourtheinfec- presentstudywasconductedtoevaluatetheinvivoinfec- tiousendogenousretrovirusRD114intheirgenome[1,2]. tivity,acuteandsubacutepathogenicity,andviralprolif- ItisknownthattheCrandell-Reesfelinekidneycelllineis