Fluorimetric Determination of Carbamate Pesticides in Host-Guest Complexes.docVIP

437 Molecules 2000, 5 Fluorimetric Determination of Carbamate Pesticides in Host- Guest Complexes Alicia Viviana Veglia INFIQC- Departamento de Química Orgánica- Facultad de Ciencias Químicas- Universidad Nacional de Córdoba- Ciudad Universitaria.- 5000 Córdoba, Argentina E-mail: aveglia@dqo.fcq.unc.edu.ar Abstract: From the effect of β-cyclodextrin and hydroxypropyl-β-cyclodextrin on the UV- visible and fluorescence spectra of carbaryl and carbofuran, the values of association con- stants were determined. The ratio of the fluorescence quantum yields for the bound and free substrates indicated an enhanced fluorimetric method of detection. Introduction The study of organized systems has proved to be very useful for analytical purposes.[1] These mi- croheterogeneous systems can be classified into: molecular aggregates (micelles, vesicles, monolayers, etc.) and polymolecular species (polysilicates such as zeolites; polysugars such as cyclodextrins (CD); polyethers such as crown ethers ). The cavities or pores with defined sizes in these permanent molecu- lar systems favour inclusion or complexing small molecules leading to high selectivity of these mole- cules. In these complexes, known as supramolecular species, are produced specific interactions that play an important role in the modification of physical and chemical properties of the substrates in- cluded.[2] Several photochemical and photophysical studies have been done with these receptors to investigate inclusion with substrates that have, for example, luminiscent properties. A remarkable in- crease of these properties was found. The molecular luminiscent spectrometry and in particular molecular fluorescence has become a routine technique with many analytical applications [3] that offers a lower detection limit and with higher selectivity than the absorption spectroscopy. Some pesticides from the carbamate group (-OCONH2) derived from fl