Formamido-Diterpenes from the South China Sea Sponge Acanthella cavernosa.docVIP

Mar. Drugs 2012, 10, 1445-1458; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Formamido-Diterpenes from the South China Sea Sponge Acanthella cavernosa Ying Xu 1,2, Jun-Hui Lang 1, Wei-Hua Jiao 1, Ru-Ping Wang 1,2, Ying Peng 2, Shao-Jiang Song 2,*, Bao-Hua Zhang 3,* and Hou-Wen Lin 1,* 1 Laboratory of Marine Drugs, Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; E-Mails: xy30490@126.com (Y.X.); zhenshanmei.hui@163.com (J.-H.L.); weihuajiao@ (W.-H.J.); wangruping_sy@ (R.-P.W.) 2 College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; E-Mail: yingpeng1999@ 3 Eastern Hepatobiliary Surgical Hospital, Second Military Medical University, Shanghai 200438, China * Authors to whom correspondence should be addressed; E-Mails: songsj99@ (S.-J.S.); zhbh_1@ (B.-H.Z.); franklin67@126.com (H.-W.L.); Tel./Fax: +86-24(S.-J.S.); +86-21(B.-H.Z.); +86-21(H.-W.L.). Received: 15 May 2012; in revised form: 1 June 2012 / Accepted: 16 June 2012 / Published: 2 July 2012 Abstract: Seven new formamido-diterpenes, cavernenes A–D ( 1–4), kalihinenes E and F (5–6), and kalihipyran C (7), together with five known compounds (8–12), were isolated from the South China Sea sponge Acanthella cavernosa. Structures were established using IR, HRESIMS, 1D and 2D NMR, and single X-ray diffraction techniques. The isolated compounds were assessed for their cytotoxicity against a small panel of human cancer cell lines (HCT-116, A549, HeLa, QGY-7701, and MDA-MB-231) with IC50 values in the range of 6–18 μM. In addition, compound 9 showed weak antifungal activity against Trichophyton rubrum and Microsporum gypseum with MIC values of 8 and 32 μg/mL, respectively, compound 10 displayed weak antifungal activity against fungi Candida albic