结构生物学sturcture biology explore-26.pdfVIP

meeting review Challenges at the frontiers of structural biology Helen R. Saibil and Elena V. Orlova m o c. A recent meeting brought together electron microscopists, crystallographers and modellers to consider the e problems facing structural biologists who wish to understand large, subcellular machines, and how the methods r u t should be extended to achieve this goal. a n . o i b t A recent Biophysical Society Discussions atomic resolution from single particle EM views must be distinguished from each c u r meeting “The structure of large biological data. The resolution of structural analysis other, and if there are variants of the struc- t s / complexes” (Asilomar, California USA, has improved significantly with the use of ture present, the problem of sorting them / : p 19–22 April, 2002) considered the prob- field emission gun microscopes, providing all out becomes very difficult. Some t t lems of determining structures of large stronger signal at high resolution. The approaches to this problem were discussed h p subcellular machines at atomic resolution. ultimate limitation for both EM and at the meeting, including matching of u Despite initial warnings about poison oak, X-ray crystallography is radiation damage. images to projections from multiple o r G ticks and earthquakes, the meeting site was This is particularly severe for cryo-EM, in models, as well as image sorting by classifi- g a pleasant California state park with pine which data collection and processing are cation to help distinguish views from n i forest and coastal sand dunes. About 1