CYP3A5基因多态性对汉族癫痫患者卡马西平稳态血药浓度及.PDFVIP

CYP3A5基因多态性对汉族癫痫患者卡马西平稳态血药浓度及 疗效的影响 ＊ # 刘建锋 ，李艳春，张国利，唐 强 （湖南医药学院附属怀化市第三人民医院，湖南怀化 418000） 中图分类号 R742.1；R968 文献标志码 A 文章编号 1001-0408（2014）36-3433-03 DOI 10.6039/j.issn. 1001-0408.2014.36.22 摘 要 目的：考察CYP3A5基因多态性对汉族癫痫患者卡马西平（CBZ ）稳态血药浓度及疗效的影响。方法：选取确诊为癫痫并 使用CBZ 治疗的患者40例，用聚合酶链反应-限制性内切片段长度多态性法检测CYP3A5基因型并分组，用高效液相色谱法测定 卡马西平的稳态血药浓度，同时观察疗效和不良反应。结果：40例患者中CYP3A5*1/* 1基因型（A 组）27例，CYP3A5*3/*3基因型 （B 组）13例。两组患者CBZ 剂量校正浓度分别为（12.39±4.13）和（16.54±3.89）μg/ml ，标准化浓度分别为（0.21±0.08）和（0.30± 0.11）μg/ml ，组间比较差异均有统计学意义（P ＜0.05）；两组患者疗效（P ＝0.726）和不良反应（P ＝0.617）比较差异均无统计学意 义。结论：CYP3A5基因多态性可能影响汉族癫痫患者CBZ 的稳态血药浓度，而对疗效和不良反应的影响尚不明确。 关键词 CYP3A5；基因多态性；卡马西平；血药浓度；疗效；不良反应 Effects of CYP3A5 Gene Polymorphism on Steady State Serum Concentrations and Therapeutic Efficacy of Carbamazepine in Chinese Han Epileptic Patients LIU Jian-feng ，LI Yan-chun ，ZHANG Guo-li ，TANG Qiang （Huaihua Third Affiliated People ’s Hospital of Hunan University of Medicine ，Hunan Huaihua 418000，China ） ABSTRACT OBJECTIVE ：To investigate the effects of CYP3A5 gene polymorphism on steady state serum concentrations and therapeutic efficacy of carbamazepine （CBZ ）in Chinese Han epileptic patients. METHODS ：40 epilepsy patients receiving CBZ therapy were enrolled in the study. Steady state serum concentrations of CBZ were measured by HPLC and their CYP3A5 genotype were determined by PCR-RFLP. Therapeutic efficacy and ADR were observed. RESULTS ：Among 40 patients ，27 patients were CYP3A5*1/* 1genotype （group A ）and 13ones were CYP3A5*3/*3 genotype （group B ）. The dose-corrected serum concentration of CBZ in 2 groups were （12.39±4.13）and （16.54±3.89）μg/ml ，and the normalized concentrations of CBZ were （0.21±0.08） and （0.30±0.11）μg/ml respectively ；there were statistical significance （P ＜0.05）. There was no statistical significance in therapeu- tic efficacy （P ＝0.726）and ADR （P ＝0.