27 237-240 z146 食品安全与检测 4+10 电导率法快速检测食品细菌总数的研究.pdf

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.2 电导率法快速检测食品细菌总数的研究 1 2 2 宁初光 ，杨洋，吴小勇 （1.无限极 (中国)有限公司，广东新会 529156 ）（2.广东药学院食品科学学院，广东中 528458 ） 摘要：电导率法是最近发展起来的应用于微生物检验的一项电化学技术。本研究先建立了利用电导率法测定几种常见食 品细菌总数的标准曲线，然后通过平行实验验证了电导率法快速检测食品细菌总数的重现性，再通过配对实验比较了电导率法 与国标法测定结果符合率；经统计学卡方检验，结果P0.05 ，表明两种方法无显著性差异，结果符合率为92.7%；说明电导率 法作为一种快速检测方法用于食品细菌总数的检验是可行的。 关键词：电导率法；细菌总数；快速检测 文章篇号：1673-9078(2012)2-237-240 Study on Conductivity Method-a Rapid Detection Method for Food Total Aerobic Bacterial Count 1 2 2 NING Chu-guang , YANG Yang , WU Xiao-yong (1.Infinitus (China) Company Ltd, Xinhui 529156, China) (2.School of Food Science, Guangdong Pharmaceutical Univeristy, Zhongshan 528458, China) Abstract: Conductivity method is a recently-developed electrochemistry technology in the microorganism detection. This study investigated the accuracy and reliability of the conductivity method on the rapid detection of food total aerobic bacterial count. At the first, three standard curves for three kinds of food were built up. And then, repeatability experiments and parallel experiments were conducted. Results indicted that there were no prominence difference between conductivity method and plate count method. The conductivity method can be used as a rapid detection method for total aerobic bacterial count in food. Key words: Conductivity method; aerobic bacterial count; rapid detection 237 收稿日期：2011-11-23 细菌总数