差分脉冲伏安法检测茶叶籽油中茶多酚含量的研究.docVIP

化学通报 2011年 hxtb@ PAGE 4 化学通报 2011年 hxtb@ 差分脉冲伏安法检测茶叶籽油中茶多酚含量的研究 庞月红?庞月红，女，31岁，副教授， ?庞月红，女，31岁，副教授，从事电分析化学及食品营养、食品安全检测方面的研究。?联系人E-mail: yhpang@; 国家自然科学基金（No.、中央高校基本科研业务费专项资金（JUSRP10918，JUSRP10920）和江南大学食品科学与技术国家重点实验室开放课题（SKLF-KF200911）资助项目 （江南大学食品学院 无锡214122） 摘 要 本文通过电化学差分脉冲伏安法建立测定茶多酚含量的新方法。选择没食子酸为茶多酚的标准品，研究没食子酸的电化学行为，实验发现没食子酸分子上的羟基在特定电位下易被氧化，产生响应电流，且没食子酸的浓度与响应电流值呈良好的线性关系，绘制标准曲线，其线性范围为0.1~1.3μg/mL，线性相关系数为0.9908，且检测的上线可以根据测定的需要继续扩大，检出限为0.04μg/mL（S/N=3）。这与茶叶及茶制品中茶多酚含量的国家标准（GB/8313-2008）相比，具有更高的检测灵敏度。该方法用于茶叶籽油中微量的茶多酚含量的测定，为1.119μg/mL。 关键词 差分脉冲伏安法 茶叶籽油 茶多酚 测定 Study on the determination of polyphenols in tea seed oil by the method of differential pulse voltammetry Pang, Yuehong * Li, Zhaoxia （State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 214122） Abstract A novel method for the determination of polyphenols in tea seed oil by differential pulse voltammetry was established. The method was based on the electrochemical behavior of gallic acid, which was the standard of polyphenols. The result showed that hydroxyl group of gallic acid was easy oxidized at the special potential. The resonance current intensity was linear with the concentration of gallic acid in the range of 0.1～1.3μg/mL and the detection limit (S/N=3) was 0.16 μg/mL. Meanwhile, the upper limit could continue expand according to practical needs. Compared with the method of HYPERLINK /dict_result.aspx?searchword=%e5%9b%bd%e5%ae%b6%e6%a0%87%e5%87%86tjType=sentencestyle=t=national+standard national standard for tea and made-tea (GB/8313-2008), this method has a higher detection sensitivity. The content of the polyphenols in tea seed oil was determined by this method and the result was 1.119 μg/mL. Key words Differential pulse voltammetry, Tea seed oil, Polyphenols, Determination 茶叶籽油是从山茶科植物茶树(Camellia sinensis O Ktze)的成熟种子—茶叶籽中所取得的可食用植物油