急性白血病患者SHP-1与c-kit基因表达及其临床意义.docVIP

急性白血病患者SHP-1与c-kit基因表达及其临床意义 作者：韩颖，罗建民，贾晓辉，王福旭，姚丽，杜行严 【摘要】 为了探讨造血细胞磷酸酶（SHP-1）基因和原癌基因c-kit在急性白血病（AL）患者中的表达及其与AL的发生及预后的关系， 应用 半定量逆转录-聚合酶链反应（RT-PCR） 方法 检测60例急性白血病患者及33名正常对照者单个核细胞的SHP-1、c-kit mRNA的表达。结果显示：SHP-1在AML细胞中表达阳性率由高至低依次为缓解组、初治组、复发组，c-kit表达阳性率则相反，各组与正常对照组相比有显著性差异（P0.05），SHP-1在AML细胞中表达阳性率比ALL细胞高，有显著性差异（P0.05），c-kit在AML细胞中表达阳性率比ALL细胞中高，有显著性差异（P0.05）；SHP-1与c-kit表达成负相关（r＝-0.502，P0.05）；30例初治AML患者SHP-1＋与SHP-1-的完全缓解率均有显著性差异（P0.05），c-kit＋与c-kit-的完全缓解率均有显著性差异（P0.05）。结论：SHP-1可能是一个潜在的抑癌基因，它负性调节c-kit基因而抑制肿瘤的发生，同时监测二者的表达可作为判断AL 治疗 转归的预测指标。 【关键词】 急性白血病； SHP-1； c-kit； 聚合酶链反应 　　Expression and Clinical Value of SHP-1 and c-kit in Acute Leukemia 　　Abstract The aim of study is to investigate the expression of hematopoietic cell phosphatase (SHP-1) gene and c-kit pro-oncogene in acute leukemia(AL) and its impact on prognosis in AL. Semi-quantity reverse transcriptase-polymerase chain reaction(RT-PCR) was used to detect the expression of SHP-1 mRNA and c-kit mRNA in 60 AL patients and 33 normal controls(NC). The results showed that the positive rates of SHP-1 expression from high to low level were found orderly in complete remission group, newly diagnosed group and relapsed group, there was significance difference between each group and NC group（P0.05）. The positive rates of c-kit expression were opposite order in each groups as compared with SHP-1. there was also significance difference between each group and NC group（P0.05）. The positive rate of SHP-1 and c-kit expressions in AML was higher than that in ALL（P0.05）, there was nagative correlation between expressions of SHP-1 and c-kit （r＝-0.502，P0.05）; The difference between the complete remission rate in SHP-1 positive and in SHP-1 negative patients from 30 newly diagnosed AML patients was significant (P005), the same result was found between c-kit+ complete remission and c-kit- complete remission. It is concluded that SHP-1 gene is a potentially anti-oncogene and inhibits the growing of tumor by negatively modulating c-kit ge