基于水稻基因组序列SSR的多态性分析.PDFVIP

( ) ( ) 中国水稻科学 Chi nese J R ice S ci , 2005 , 19 4 :303 ～307 ht tp :/ / www . ricescience . or g 303 基于水稻基因组序列 SSR 的多态性分析 陈仲中　汪旭升 　朱 　军 (浙江大学 生物信息学研究所 , 浙江 杭州 3 10029 ; Email :zhongzhongchen @sohu . com ; 通讯联系人 , Email : j zhu @zj u . edu . cn) A naly si s of SSR Polymorp hi sm by GenomeScale Comp aring Bet ween Varietie s in Rice CH EN Zhongzhong , WAN G Xusheng , ZHU J un ( I ns t i t ute of B ioi nf orm at ics , Z hej i ang Uni vers ity , H ang z hou 3 10029 , Chi na; Em ai l :z hongz hongchen @s ohu . com ; Corresp on di ng aut hor , ) Em ai l : j z h u @zj u . e d u. cn Abstract : One t hou sand four hundred and fift y t hree loci were identified t he same a s in t wo rice varieties , indica rice 9311 and j aponica rice Nipponbare , by u sing bioinfor matics tool s based on 6655 sequences relea sed by Mon santo ent erp ri se which con si st ed of p erfect rep eat s flanked by 100 bp on eit her side of t he simple sequence rep eat ( SSR) . Among t ho se sequence hit s , 1449 and 145 1 SSRs were det ect ed in 9311 and Nipponbare , r esp ectively . Among t ho se , 37 1 SSRs had t he same rep eat unit s and t he same rep eat count , while 804 SSRs had t he same sp ecial motif s wit h differ ent rep eat count s . In addition , in t he same rep eat unit s wit h different rep eat count s SSR cla ss ,dinucleotide SSRs were t he mo st abundant , occupying 62 . 94 % identified , while p ent a and hexanucleotide SSRs were t he least f requent . Poly