微流控免疫芯片富集捕获EHECO157︰H7的研究-第三军医大学学报.doc

微流控免疫芯片富集/捕获EHEC O157︰H7 摘 要：目的　探讨基于免疫微珠的微流控芯片识别捕获EHEC O157︰H7EHEC O157︰H7μm的玻璃微珠上，再将微珠填充于PDMS/玻璃复合芯片μl/min和5min的最佳冲洗流速和冲洗时间下，该芯片在10min内可完成对EHEC O157︰H7EHEC O157︰H7 关键词：微流控芯片；免疫微珠；细菌捕获 中图法分类号：Q93-332；Q93-338 文献标识码：A A microfluidic chip to concentrating and capturing EHEC O157︰H7Department of Analytical Chemistry, College of Laboratory Medicine, Third Military Medical University, Chongqing 400038, China) Abstract: Objective To study the efficiency of concentration and capture of enterohemorrhagic Escherichia coli (EHEC) O157∶H7 by a self-made microfluidic chip based on mic