应用SELDI-TOF-MS技术筛选卵巢癌淋巴结定向高转移特性细胞株差异表达蛋白.pdfVIP

《癌症 Chinese Journal of Cancer , 2ω8， 27(1 1):1155…IIω 1155 ·基础研究· 应用 SELDI-TO札MS 技术筛选卵巢癌淋巴结 定向高转移特性细胞株差异表达蛋白 冠潇，黎丹戎，正琪，李力，阮和云，张炜 Screening of Differentially Expressed Proteins Associated with Directional Highly Lymphatic Metastasis in Ovarian Carcinoma Cell Lines Using SELD卜丁OF帽 MS 丁echnology GUAN Xiao , LI Dan-问ong ， WANG Qi , LI Li, RUAN He.…Yun , ZHANG Wei [ABSTRACT] BACKGROUND OBJECTIVE: The ovarian serous papillary 广西医科大学附属肿瘤医院 adenocarcinoma cell line SKOV3 and its subclones SKOV3-pm2 and SKOV3- 妇瘤科， pm3 are cell models to investigate the molecular mechanism of invasion and 广西南宁 53∞21 metastasis 01 ovarian cancers. This study was to screen differentially expressed proteins between ovarian carcinoma cell lines with directional D叩耐ment 01 Gynecological (SKOV3-pm2 and SKOV3-pm3) and non-directional (SKOV3) highly Oncology , Iymphatic metastasis potentials using time-of-flight mass spectrometry Gu棚.gxi Medical technology and protein chips. METHODS: The Iymphatic metastasis rates of University , the three cell lines were detected in animal models. Proteins in endochylema Nanning , G阳1酬， 53ω121 and supernatants of the three cell lines were screened using surface- P. R. China enhanced laser desorption and ionization-time 01 flight翩 mass spectrometry (SELDI-TO札MS). Each sample was examined using weak cation exchange