转染纤溶酶原激活剂抑制剂22基因对纤维肉瘤细胞侵袭能力的影响.PDF

3·56 · Ch in J O nco l, Sep tem ber 1996, V o l 18, N o. 5 转染纤溶酶原激活剂抑制剂2 基因 对纤维肉瘤细胞侵袭能力的影响 曹祥荣　章　玲　苏长青　王革平　戴君勇　徐贤秀　陈宜峰 摘要　作者将转染纤溶酶原激活剂抑制剂2 (PA I2) 基因的纤维肉瘤细胞克隆和对照细胞铺在 3 ( ) 同位素标记的H 细胞外基质 ECM 上, 液体闪烁计数法测定释放到培养液中的同位素。并进行裸小 鼠皮下接种肿瘤细胞和组织学研究, 以了解 PA I2 在肿瘤细胞侵袭过程中的作用。结果表明PA I2 可抑制肿瘤细胞降解3 能力, 抑制率达 865% 。 染色种植瘤切片, 光镜观察显示, 在实验组 H ECM H E 瘤组织的周围有加厚的包膜, 而对照组瘤组织旁无明显的包膜。包膜是肿瘤细胞侵袭的障碍, 因此, 重 组 PA I2 可抑制纤维肉瘤细胞的侵袭能力。 主题词　纤维肉瘤　　纤溶酶原激活物抑制物 2　　细胞外基质　　包膜 Invasion of f ibrosarcoma cells tran sfected with PA I- 2 gene 　Cao X iang rong , Z hang L ing , S u Chang qing , et al. D ep artm ent of B iology , N anj ing N orm al U niversity , N anj ing 210097 Abstract　T he effect of PA I2 on the invasion of fibro sarcom a cells in vitro and in vivo w as in . ( + 、+ ) 2 ( + ) vestigated T he contro l cells C C pem and PA I transfectants C exp w ere used in the assay 3 . of the degradation of iso top ically labelld H ECM and w ere injected sc into athym ic nude m ice R e 2 3 ( 86. 5% ). com binant PA I could inh ibit efficiently the degradation of H ECM by tumo r cells T he PA I2 transfectants rem ained tumo rigenic in nude m ice, but tumo rs o riginating from the PA I2 trans fectants show ed h isto logically the p resence of a th ick cap sule w h ich w as absent in tumo