生后早期听觉剥夺对大鼠听皮层NR2B mRNA年龄——依赖性表达模式影响.pdfVIP

The effect of early auditory deprivation on the age-dependent expression pattern of NR2B mRNA in rat auditory cortex Caixia Bi, Yilei Cui, Yuting Mao, Jinghong Xu,Feng Xu,Jiping Zhang, Xinde Sun* Research Centre for Brain Science, East China Normal University, Shanghai 200062, China Abstract Using in situ hybridization (ISH), present research minutely investigated the age-dependent expression pattern of NR2B mRNA in rat auditory cortex (AC) during postnatal development and the effect of early auditory deprivation. For the rats in the normal group, the mRNA expression of NR2B was highest at birth (postnatal day 1), but declined rapidly until low level of adulthood, during which there was a rising period with a transient peak on postnatal day 21 (P21); for the rats in the deprived group, the whole declining-as-aging trend of NR2B mRNA expression was similar to that of normal group, but the expression level from P15 to P27 were much lower than that of normal group with the greatest reduction from P21 to P23 and the transient peak on P21 disappeared. In both groups, the distribution pattern of NR2B mRNA positive neurons in 6 layers and dorsal, medial and ventral sub-districts of auditory cortex was also examined. There is no significant effect on the expression pattern between normal and deprived group. Our results indicated that the early auditory deprivation decreased the age-dependent expression pattern of NR2B mRNA, suggesting that NR2B may play an important role in the developmental plasticity of auditory function in rats. Keywords: Auditory Cortex; Auditory Deprivation; In situ hybridization (ISH); NR2B mRNA Expression 1. Introduction During a sensitive period in postnatal days, animals show