HIV_1感染对T和B细胞核内UNG2mRNA表达影响_张驰宇.pdf

DOI牶牨牥牣牨牫牫牨牪牤j牣issn牣牨牰牱牨牠牱牱牳牫牣牪牥牥牱牣牥牨牣牥牥牪 17 1 ( ) Vo.l 17 No. 1 　 2007 1 Journal of Jiangsu University(M edicine Edition) 　 Jan. 2007 　 　 　 　 　 　 HIV-1 T B UN 2 mRNA 1, 2 1 2 张驰宇 , 许燕萍 , 贲昆龙 (1. , 212013;2. , 650223) [　]　: HIV-1UN 2。: SYBR greenⅠ RT- PCR, HIV-1 T B , HIV-1 C8166 UN 2 mRNA 。: UN 2mRNAH IV-1 T HIV-1 C8166, 8. 76 8. 14, HIV-1 B。:HIV-1 UN 2, TCR Th, UN 2。 []　DNA;H IV-1;T;; []　Q5;R512. 91　　[]　A　　[]　1671- 7783(2007)01 -0005- 04 Up-regulation of human nuclear form of uraci-lDNA glycosylase mRNA in HIV-1 infected human peripheralT cells and C8166 cells 1, 2 1 2 ZHAN G Ch -iy u , X U Yan-p ing , BEN K un-long (1. School ofM edical Technology, Jiangsu University, Zhenjiang Jiangsu 212001;2. Kunming Institute of Zoology, ChineseAcademy of Sc-i ences, Kunm ingYunnan 650223, China) [A stract]　O jective:To investigate whetherH IV-1 infection m ay activate the expression of nuclear UN 2mRNA ofT and B cells, which may inhibit antibody gene hypermutation and decrease antibody d-i versity. M ethods:The4-step program in SYBR greenⅠ rea-l tmi e RT-PCRwasused to quantify the expre-s sion of UN 2 mRNA in T and B cells ofH IV-1-infected individuals, and inH IV-1-infected C8166 cells. Results:The expression of UN 2 mRNA in T cells from HIV-1 infected patients and in C8166 cells infec- ted w ith H IV-1was found to be obviously up-regulated. Their relative expression indices were 8. 76 and 8. 14 in comparison w ith those of uninfected controls, respectively. Conclusion:The increased UN 2 i