HPLC测定保健食品中微胶囊型维生素A.PDF

HPLC 测定保健食品中微胶囊型维生素 A  1 2 1 1 颜琳琦 ，戚绿叶 ，张丽媛 ，陶巧凤 (1.浙江省食品药品检验研究院，杭州 310004 ；2.浙江工业大学药学院，杭州 310014) 摘要：目的 建立测定保健食品中微胶囊型维生素A 含量的反相高效液相色谱法。方法 以适宜浓度氨水溶液破坏保健 食品中微胶囊型维生素A 的微胶囊结构，再以有机溶剂提取。采用Agilent ZORBAX SB-C18 色谱柱(250 mm×4.6 mm ， 1 1 5 µm) ，以甲醇为流动相，流速1.0 mL·min ，检测波长为325 nm ，柱温35 ℃。结果 维生素A 在 1.41~56.4 µg·mL 内 线性关系良好(r=1.000) ；平均回收率为 97.5%(RSD=1.9%)。结论 本法方法简单、准确，重现性好，适用于保健食品中 微胶囊型维生素A 的测定。 关键词：高效液相色谱；维生素A ；微胶囊；保健食品 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2014)07-0860-04 DOI: 10.13748/ki.issn1007-7693.2014.06.023 Determination of Vitamin A in the Form of Microcapsule in Functional Food by HPLC 1 2 1 1 YAN Linqi , QI Lüye , ZHANG Liyuan , TAO Qiaofeng (1.Zhejiang Institute for Food and Drug Control, Hangzhou 310004, China; 2.College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China) ABSTRACT ：OBJECTIVE To develop an HPLC method for determination of vitamin A in the form of microcapsule in functional food. METHODS Microcapsule structure was disrupted by suitable ammonia solution and vitamin A was extracted by organic solutions. The chromatographic separation was achieved on an Agilent ZORBAX SB-C 18 column(250 mm×4. 6 mm, 5 µm) with methanol as the mobile phase. The detection wavelength was at 325 nm, flow rate 1.0 mL·min1 and the column temperature 35 ℃. RESULTS The linear range of vitamin A was 1.4156.4 µg·mL1(r=1.000). The average recovery of vitamin A was 97.5%(RSD=1.9%). CONCLUSION The established method was simple, accurate and with