鲫鱼卵中脂质提取方法的比较.pdfVIP

第 17 号在第 l 期 上海水产大学学报 Vo1. 17 , No.l 2008 年 1 月 JOURNAL OF SHANGHAI FISHERI航S UNIVERSITY Jan. ， 2ω8 文章编号: 1004 -7271 (2008 )01 - 0104 …05 自ø~ 鱼卵中脂质提取方法的比较 陆丽丽，陈舜胜 (上海水产大学食品学院，上海 2∞090) 摘 堕:比较了提取酬鱼卵的脂质和磷脂的三种方法。酶水解唰乙醉法提取的脂质和磷脂的颜色较油、得率较 高，提取的磷脂主要成分为磷脂毗阻碱;正己脱乙醇幽丙酬法提取总磷脂、富含朋醇的中性脂和含少量固醇类 的中性脂，两酬洗涤能有效地去除磷脂中的中性脂，特别是易榕子乙醇的固醇和三瞄系醇，最低限度地使用有 毒有机悔剂;而Bligh-Dyer 提取总脂后液液萃取的经典方法提取脂质虽也较简便，但其大量使用有毒榕剂，安 全性低。此外，用回事单榕剂、洗脱，紫外检测器在线检削进行的低压柱层析，分离正己皖w 乙醇分提的磷脂，制备 了较高纯度的磷脂酷胆碱和磷脂酷乙醇膛。 关键词:侧像卵;脂质;磷脂;提取;比较 中团分提号:TS 20 1. 2 文献标i只码:A Comparison on methods of extracting lipids from roes of Cαrassius auratus LU Li-li , CHEN Shun-sheng (College 01 Food Science Technology , Shanghai Fisheries University , Shαnghai 200090 , China) Abslracl: The color and yield of the lipids and phospholipids were better by enzymatic hydrolysis and ethanol extraction method , with PC as the main component in the extracted pho日pholipids. Method of. hexane and ethanol extraction and then washing by acetone could easily and efficiently extract total phospholipids , and the neutral lipids rich in 的terols and another neutral lipid日 with lower sterols. Acetone washing could remove the neutral lipids from the phospholipids , especially the sterol and triterpenoic alcohols which are easily dissolved in the alcohol , and only a little poisonous organic solvent was used. Though the classic method Bligh-Dyer is handy , the large amount of noxious solvent it used made it unsafe. In addition , higher purity of PC and PE are prepared from the hexane and ethanol extracted phospholipids by lo