E-electin在大肠癌的表达及其在血行转移中的作用初探.pdfVIP

黛庆医科大学学报2(刷得第34 卷第7 期 (Joumal of Chongqing Medical University 2刷贼 Vol.34 NO.7) 甲町 867 …帽 肿瘤分子学相关研究，文章编号:0253-3626(2酬)07-0867-03 E-selectin 在大肠癌的农达及其在血行转移中的作用切探 郎伟思，正继见，张杨眉 (重庆医科大学附属第二医院普外科，重庆 4创XHO) [摘 噩}目的:研究细胞粘附分子b附lectin 在大肠癌组织中表达及其在大肠癌血行转移中的作用。方法:应用免疫组织化学 方法枪测大肠癌组织和正常大肠组织E-selectin 的表达;并利用Transwell 小京分析人大肠癌细胞株在不同浓度 E-selectin 时的 转移能力变化。结采:E-selectín 在大肠瘤细钥表达的阳饨率为 57.5%，显著高于正常大肠组织的 6.67%(PO.05); 有远处转移 者阳性率90%跟著商于无远处转移者的 46.67% (PO.05 );随着 b附lectín 浓度升高(l μ圳Jμg1L， IOμg1L)，大肠癌细胞穿 腰数目增多[(27.2 土 4.1 3) 、 (52.9 士 3.56) 、 (1∞.7 土 8.15)]。结论:E-附lectin 在大肠蜡提高表达并可能在其血行转移中发挥了一定 的作用。 {关键诩}细胞帖附分子 E-selectin{E- 选择素);大肠捕;转移 {中罔周书分提法分类号]R735-34 (3t献栋惧码] A (收稿日期}2∞8-11-27 Expression of E…selectin in colorectal neoplasm and its role in the metastasis of blood line LANG Wei叫si ， etal (Depαrt阳nt of General Surgery , the Second Affiliated Hospital , Clwngqing Medicial University) [Abstract] Objective: To investig刷刷啊。1 of cell adhesíon molecule E-selectin ín colorecta.I neopwm and i凶 relations wi也 meta.stasis in the blood line. Me仇。ds:E-selectin expression in colorectal carcinorna and nonnal COlO1曹ctal tissue were detected wi出 immunohisochemical technique. The change of shi仇 ahility of human colorecta.I neoplasm cellline w郁郁wyzed. 如sults: The expressing mteofE-….selectin was 57.5~昏， obviously higher than 6.67~毛 in the normal colorectal 伽sue( PO.05); Wi伽 the incr.础se in cancentraction of E-扉electin (1μg1L， 5μgIL and IO ~ι gIL ), the number of intestineωn(，er c咽lls penetracting the membrane increased large [(27.2 士 4 .1 3) ,(52.9 士 3.56) ，(1拥.7 我 8.1 5)] (PO.05 ). Conclus切n: E-selectin in the colorectal neoplasm assumed is highly expressed possibly and play自附民ain role in the metastasis of blood line. [Key words ]Cell adhesion mole呗u1e 阳lectin E(ιselec