three expression systems-main：发酵的三大表达系统.pdf

Journal of Biotechnology 127 (2007) 335–347 Review Select what you need: A comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes Jiechao Yin a , Guangxing Lib , Xiaofeng Ren a,∗, Georg Herrler c a Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, China b Department of Basic Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, 150030 Harbin, China c Institute for Virology, University of Veterinary Medicine Hannover, Buenteweg 17, D-30559 Hannover, Germany Received 31 October 2005; received in revised form 13 July 2006; accepted 20 July 2006 Abstract The expression of heterologous proteins in microorganisms using genetic recombination is still the high point in the devel- opment and exploitation of modern biotechnology. People can produce bioactive proteins from relatively cheap culture medium instead of expensive extraction. Host cell systems for the expression of heterologous genes are generally prokaryotic or eukary- otic systems, both of which have inherent advantages and drawbacks. An optimal expression system can be selected only if the productivity, bioactivity, purpose, and physicochemical characteristics of the interest protein are taken into consideration, together with the cost, convenience and safety of the system itself. Here, we concisely review the most frequently used prokary- otic, yeast, insect and mammalian expression systems, as well as expressio