黑穗醋栗果实多糖清除自由基活性及结构初步研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.12 黑穗醋栗果实多糖清除自由基活性及结构初步研究 徐雅琴，宋秀梅，任中杰，邵铁华，张鑫 （东北农业大学理学院，黑龙江哈尔滨 150030） 摘要：以黑穗醋栗果实为原料，分别用80%、70%、60%、50%、40% 、30%乙醇醇沉并采用D4006 大孔树脂初步纯化制备6 种多糖。分别进行DPPH·、OH·和O2-·清除试验，结果表明6 种多糖对自由基均有不同程度的清除作用，其中80%醇沉制备的多糖 （BCP-1）对三种自由基的清除作用最强。利用葡聚糖凝胶SephadexG-100 对BCP-1 纯化制备均一组分BCP-1- Ⅱ，采用气相色谱法 和高效液相色谱法对BCP-1- Ⅱ进行单糖组成分析及分子量测定。结果表明：BCP-1- Ⅱ分子量为51880 u，并且由4 种单糖组成，摩尔 比为阿拉伯糖:甘露糖:葡萄糖:半乳糖=9.08:1.00:15.77:0.41。红外光谱扫描结果表明其含有多糖的特征吸收峰，并且可能含有吡喃糖环 及α-糖苷键。 关键词：黑穗醋栗；多糖；自由基；分子量；单糖组成 文章篇号：1673-9078(2013)12-2821-2825 Analysis of Structure and Free Radical Scavenging Activities of Ribes nigrum Polysaccharides XU Ya-qin, SONG Xiu-mei, REN Zhong-jie , SHAO Tie-hua, ZHANG Xin (College of Science, Northeast Agricultural University, Harbin 150030 , China) Abstract: Six kinds of crude polysaccharides were precipitated with 80%, 70%, 60%, 50%, 40% and 30% ethyl alcohol respectively from Ribes nigrum fruit. Then the DPPH ·, OH ·and O2- ·scavenging activities were investigated after purified by macroporous resin D4006. The results showed that six kinds of polysaccharides had certain scavenging activities and the polysaccharides precipitated with 80% ethanol (BCP-1) had stronger free radical elimination capacity. Homogeneous component (BCP-1- Ⅱ) was prepared after further purification from BCP -1 by Sephadex G-100. The compositions of the main fraction BCP-1- Ⅱ were identified and quantified by GC and HPLC. Results indicated that BCP-1- Ⅱhad an average molecular weight of 51880 u and contained arabinose, mannose, glucose and galactose with the molar ratio of 9.08:1.00:15.77:0.41. Characteristic absorption of polysaccharide was shown in IR spectrum and the monomer o