OIE手册-牡蛎疱疹病毒概述.pdfVIP

NB: Version adopted by the World Assembly of Delegates of the OIE in May 2014 C H A P T E R 2 . 4 . 9 INFECTION WITH OSTREID HERPESVIRUS 1 MICROVARIANTS 1. Scope For the purpose of this chapter, infection with ostreid herpesvirus 1 microvariants is considered a viral infection of bivalve molluscs caused by ostreid herpesvirus 1 microvariants. Microvariants of OsHV-1 (ostreid herpesvirus 1) are genotypes of OsHV-1 that have sequence variations in a microsatellite locus upstream of the ORF4 and in ORF4 and ORF42/43 when compared with the reference sequence (accession number AY509253). The term “microvariants” is used in this chapter to refer to µVar and related variants. The term µVar is used to define a single variant presenting all the mutations reported by Segarra et al. (2010). Mortality associated with OsHV-1 microvariants has been reported in the Pacific oyster, Crassostrea gigas, and in the Portuguese cupped oyster, C. angulate. 2. Disease information 2.1. Agent factors OsHV-1 is the aetiological agent of a contagious viral disease of Pacific oysters, also affecting other bivalve species. The genome of the virus was sequenced from infected Pacific oyster larvae collected in France in 1995 (Davison et al., 2005). As this specimen was the first to be described (through complete genome sequencing, accession number AY509253), it can be considered the reference type. 2.1.1. Aetiological agent, agent strains OsHV-1 particles have been purified from Pacific oyster larvae in France (Le Deuff Renault, 1999) and were observed by transmission electron microscopy to be enveloped icosahedral with electron dense cores and a diameter around 120 nm. The intranuclear location of t