抑菌试验(国外英文资料).doc

抑菌实验(国外英文资料) 4, bacteriostasis test The Optochin sensitive test? ? (1) the principle: Optochin is the name of the product of ethyl hydroxylhydroxyl hydrochloride. There is a specific inhibition of streptococcus pneumoniae, which may interfere with the biosynthesis of folic acid, but not other streptococcus. (2) methods: cotton swab to influenza virus bacteria broth cultures evenly coated on blood AGAR plate, with a piece of paper containing 5 mu g the barleycourt hin, candle cylinder or carbon dioxide incubator, 35 ℃ 18 ~ 24 h incubation, observations. (3) results: the diameter of the bacteriostatic ring is greater than 14mm, less than or equal to 14mm negative. (4) application: mainly used in the identification of streptococcus pneumoniae (sensitization) and other streptococcus (drug resistant). The bacterium is sensitive to the bacterium ? (1) principle: group A streptococcus is almost 100% sensitive to bacillus, while other groups of streptococcus bacteria are usually resistant to bacillus. The test can be used to identify streptococcus. (2) methods: cotton swab to influenza virus bacteria broth cultures evenly coated on blood AGAR plate, slightly dry after posted a 0.04 U of bacitracin paper and 35 ℃ 18 ~ 24 h incubation and observations. (3) results: the diameter of the bacteriostatic ring is greater than 10mm and less than 10mm for resistance. (4) application: mainly used in the identification of group A and non-a group streptococcus. 5 to 15 percent of the isolated strains of the strain were also sensitive to bacillus, such as 6 percent of group B streptococcus, 7.5 percent of group C streptococcus, and G group streptococcus. 3. O / 129 test Principle: (1) O / 129 (2, 4 diamino - 6, 7 - diisopropyl with organism) can inhibit vibrios, luminous bacillus genus and adjacent bacterial growth, the bacterium aeromonas and pseudomonas bacteria resistant. (2) methods: cotton swab to quarantine fungus bacteria suspension uniformly coated on the alkaline AGAR plate, wi