植物性食物中青霉素类兽药残留测定功课领导书)(国外英文资料).docVIP

动物性食品中青霉素类兽药残留测定作业指导书)(国外英文资料) Instructions for the test of the residue of penicillin in animal food One, a brief introduction The guidance book is applicable to the residual determination of penicillin in animal food. Second, reference standards Ministry of agriculture 958 announcement -7-2007 pig chicken edible tissue of the penicillin class drug residue detection method Ministry of agriculture 1163 notice -5-2009 animal food in the animal food products of ampicillin residue detection The department of agriculture announced the determination of the residual amount of penicillin in milk from 781-11-20 NY/T 829-2004 the residual test method for ampicillin residue in milk Third, the operation step The preparation of a blank matrix solution: taking blank sample 5.00 (+ + 0.05) g, with 3 operations, parallel 3. 2. The preparation of the reference substance solution: precision said take penicillin kind of veterinary drug products is (penicillin V potassium, sodium penicillin G, ampicillin, benzene (Westwood) made standard stock solution, right amount water and diluted into a standard working solution of 0.01 mg/mL. The preparation of the sample solution: 3.1 extraction precision according to take samples (5 + / - 0.05) g put in 50 ml polypropylene centrifugal tube, adding phosphate buffer 15 ml, oscillation, 5 min and mixed with n-hexane 5 ml, oscillation blending, 3000 r/min, the centrifugal 15 min, abandon to n-hexane layer, transfer layer to heart in a bottle; Then the phospho-buffer 10ml and n-hexane 5ml were repeated for 2 times, removing the n-hexane layer and the three extracts for SPE purification. 3.2 extraction purification The C18 solid-phase extraction column was activated by 3 mL of methanol, water, sodium chloride solution and phosphate buffer, and extracted fluid. 1 ml water and acetonitrile ml elution, collect the eluent, in 45 ~ 50 ℃ dry nitrogen. 3.3 derivation reaction Eluent in 45 ℃ nitrogen blow dry, add 1 ml vortex vibration dissolve standard dilu