3-大豆苷元磺酸钠对氧化低密度脂蛋白致内皮细胞损伤的保护作用研究.docVIP

3-大豆苷元磺酸钠对氧化低密度脂蛋白致内皮细胞损伤的保护作用研究 作者：曾靖， 黄玉萍， 黄玉珊， 王树人 【摘要】 目的研究3’-大豆苷元磺酸钠(DSS)对氧化低密度脂蛋白诱导脐静脉内皮细胞损伤的保护作用及其机制。方法体外培养脐静脉内皮细胞，将细胞分为空白对照组、氧化损伤对照组(ox-LDL)、氧化损伤加入维生素E对照组(VE+ox-LDL)、氧化损伤加入DSS低、中、高浓度组(DSS-L、DSS-M及DSS-H组)。将ox-LDL作用于预先加入VE及不同浓度DSS预处理24 h的内皮细胞，继续培养24h，MTT法检测细胞活力，比色法检测丙二醛(MDA)及一氧化氮(NO)的含量，检测乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)及谷胱甘肽过氧化物酶(GSH-Px)的活性。结果预先加入VE、中高浓度的DSS可提升损伤的内皮细胞的活力，可显著降低损伤内皮细胞的MDA及NO的释放，可显著提升损伤细胞的LDH、NOS、SOD和GSH-Px的活性，但低浓度的DSS对上述指标影响不大。结论DSS可减轻ox-LDL对血管皮细胞的氧化损伤，起到一定的抗氧化保护作用。 【关键词】 3’-大豆苷元磺酸钠; 氧化性低密度脂蛋白; 内皮细胞; 保护效应 　　Abstract：ObjectiveTo investigate the protective effect and mechanisms of 3’-daidzEin sulfonate sodium on vascular endothelial cell injury induced by oxidized low-density lipoprotEIn. MethodsHuman umbilical vein endothelial cells(HUVECs) were cultured in vitro and divided into 5 groups:control，ox-LDL, VE+ox-LDL, DSS-L，DSS-M and DSS-H groups. Endothelial cells were incubated with ox-LDL for 24 hours after they were incubated with Vitamin E(VE) and different doses of DSS for 24 hours. The proliferation of HUVECs was assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method，and the levels of NO and MDA，the activities of LDH, SOD, NOS and GSH-PX were measured. ResultsThe level of MDA and the activity of LDH in ox-LDL damaged group were remarkably higher than those in VE，DSS-M and DSS-H groups. The activities of SOD，NOS，GSH-PX and the concentration of NO in ox-LDL damaged group were lower than those in VE，DSS-M and DSS-H groups. There was no significant difference between DSS-L group and ox-LDL group. Conclusion3’-daidzein sulfonate sodium has protective effect on HUVECs injury induced by ox-LDL，which may be related to increasing the antioxidant capacity of HUVECs. 　　Key words：3’-daidzein sulfonate sodium; Oxidized LDL; Endothelial cell; Protective effect 　　血管内皮是介于血液与血管壁组织之间的一层上皮组织，它不仅是一种机械屏障，还具有物质转运、自分泌、旁分泌等多种功能，在创伤修复、血管生成、止血、血栓形成、动脉粥样硬化等一系列生理和病理过程中起着重要作用。氧化低密度脂蛋白(ox-LDL)所致内皮细胞损伤是动脉粥样硬化形成的一种重要因素，寻找有效的抗氧化剂防止血管内皮