牛鲍计数板(国外英文资料).docVIP

牛鲍计数板(国外英文资料)1 reagents:The red blood cell (Hayem fluid) :Sodium chloride (NaCl) 1.0 g, sodium sulfate crystallization (Na2S04 10 h20) 5.0 g or anhydrous sodium sulfate (Na2SO:) 2.5 g, mercuric chloride (HSCl:) 0.5 g, distilled water added to 200 mi.[specimen]End blood or EDTA - K3 (1.5 mg/ml full blood) of the anticoagulant blood, measured in 4 hours.[operation]Take a small test tube and add a red cell diluent 2.0 mi.Remove 10 mu l of blood from the end with a clean, dry micropipette.Wipe out the outside of the tip of the tube, gently blow into the base of the red blood cell, then gently absorb the top and rinse the straw two to three times, then mix well immediately.4. The pool for 2 to 3 min, after filling in at high magnification in counting the big box of four corners and in the middle of 5 box of red blood cells, according to the number without number, for the left without visual counting number right principle. Must follow a certain direction when counting one by one to avoid repeat or omission.Finish the count, rinse the counting pool and cover glass with distilled water in time, wipe dry with fine cotton cloth, avoid using coarse cloth, and wipe with coarse paper.6. Calculation:The red blood cell count/L is equal to N times 5 times 10 times 201 times 10, 6= N present 100 x 10 of 12Type:N represents the number of red blood cells in the five squaresThe number of red blood cells in 5 is converted to 1Grid cell numberX 10 is the number of red blood cells in the blood cell of 1.0 muThe dilution of the bloodX 106 is converted by mu l to l[reporting]The number of red cells is X.X X times 10, 12 / L[methodology]Using blood count plate for cell count is one of the most commonly used basic skills for medical students, although now has a variety of automated analysis method, but due to the classic, the method is convenient, practical, are still applied to clinical examination and scientific practice. Visual technique as main source of error: equipment is not accurate,