蔬菜生果中维c含量的测定(国外英文资料).docVIP

蔬菜水果中维c含量的测定(国外英文资料) Shake well. Add 4mL after 20min. 10% HCl, mixed, and fixed to the scale. The absorbance is measured at the maximum absorption wavelength by the vacuum of distilled water 1.2.6? The calculation of vitamin C content After measured the absorbance of fruit and vegetable samples, according to the standard curve equation, to calculate the vitamin C content in fruit and vegetable [mg · (100 g) - 1]. Vitamin C = C * V V is the total for the total x 1000 x 100 v1 x W type: C is in accordance with the standard curve equation to calculate the concentration of vitamin C, mu g · mL - 1; So V1 is the volume of the sample solution for the absorbance. The total volume of the sample is determined by V. The total volume of V is for the sample. W is always the quality of fruits and vegetables, g; 100 grams of fruit and vegetables 2? The results and discussion 2.1? Absorption curve ( FIG. 1) in figure 1 ? Vitamin C absorption curve Fig. 1? What? The curve? Of? Vitamin? C? Solution the figure 1 shows that in 200 ~ 300 nm wavelength range, vitamin C has a maximum absorption peak, maximum absorption wavelength of 243.4 nm. Therefore, this The experiment selected 243.4 nm for wavelength 2.2 standard curve (FIG. 2) the figure 2 shows that in 1.0 ~ 12.0 mu concentration range, g. mL - 1 vitamin C concentration and absorbance conforms to the lambert beers law, a good linear relationship. The linear regression equation is A = 0.0528 c-0.0568, the correlation coefficient R2 = 0.9996 The choice of static time is influenced by the unstable and easily decomposing of vitamin C in the alkaline medium, which affects the measurement of absorbance. The experiment found that the resting time of the extracted liquid was related to the extraction quantity. In the case of green pepper (the largest sample of 1.0 mL), after adding lye, the relationship between static time and absorbance at room temperature was investigated, and the results were shown in figure 3 It is shown in figure 3