杞黄颗粒中挥发油提取和β-CD包合工艺的研究.docVIP

杞黄颗粒中挥发油提取和β-CD包合工艺的研究 作者：钟华，尹蓉莉，周淑芳，张丹，李毅 【摘要】 　　目的考察杞黄颗粒中挥发油的最佳提取工艺和β-CD最佳包合工艺。方法采用单因素以收油率作为评价提取工艺的指标；以包合物收得率和挥发油包合率的综合评分作为评价包合工艺的指标优选β-CD包合工艺。结果挥发油最佳提取工艺为：莪术加水6倍量，浸泡12 h，提取6 h；挥发油的最佳包合工艺为采用饱和水溶液法在一般温度下β-CD与挥发油的配比为61，水与挥发油的配比为601，包合时间为45 min。结论所选提取工艺提取率高，包合工艺合理可行。 【关键词】 杞黄颗粒； 挥发油； β-CD包合物； 单因素实验； 正交实验 　　Abstract：ObjectiveTo study the optimum conditions for the extraction of volatile oil in Qihuang Granule，and for the preparation of thEir β-CD inclusion compounds．MethodsThe study was carried out with single factor .With the percentages of extracted volatile oil as the target markers,the optimum conditions was determined for extraction of the volatile oil．With the ratio of inclusion and the oiling bearing from the inclusion as the target marker the conditions for including the oils with β-CD were optimized．ResultsThe optimized extraction conditions were：the water was 6 times of the herbs in wEIght，the herbal medicine of Rhizoma Curcumae should be extracted for 6 h after advanced soaking 12 hours with water．The optimal inclusion conditions were：applying the saturated aqueous solution method, under the nature temperature, the proportion of β-CD and oil was 6 g：l ml, the proportion of water and oil was 60 ml：l ml , the time was 45 minutes，respectively．ConclusionThe established process can be used for the extraction and the inclusion of volatile oil in Qihuang Granule．The obtained ratio of oil is high，the inclusion process is suitable． 　　Key words：Qihuang Granule； Volatile oil； β-CD inclusion compounds； Single factor test； Orthogonal design test 　　杞黄颗粒是由黄芪、枸杞、莪术、泽泻等中药制成的复方制剂，具有益气血、补肝肾、活血化淤的功效，多用于 治疗 年老体虚，气血不足，肝肾亏损引起的气血淤滞。方中莪术和泽泻均含有挥发油，尤其是莪术挥发油在发挥功能主治方面起重要作用，所以需要考虑挥发油的提取和包合问题。本实验采用单因素实验对挥发油提取工艺进行了研究，并采用正交实验对β-环糊精(β-CD)包合工艺进行了优选。 　　1 仪器与试药 　　BP211DAG 电子 天平(德国Sartorius)，SHZ型循环水真空泵(上海亚荣生化仪器厂)，KDM型控温电热套，DL-720D型数控超声波仪(昆山市超声仪器公司)，挥发油测定器(上海玻璃厂)；莪术、泽泻药材(五块石中药材市场购买，经成都中医药大学中药鉴定教研室鉴定均符合《 中国 药典》2005年版规定)。β-环糊精(含量99％，中国医药集团上海化学试剂公司)；水为重蒸馏水，无水乙醇、石油醚(沸程30～60)等其它化学试剂均为分