模拟酸雨对菲律宾樟树幼苗抗氧化系统的影响.docxVIP

En-U LiuChiung-Pin Liu.Effects of Simulated Acid Rain on the Antioxidative System in Cinnamomum philippinense Seedlings[J].Water Air Soil Pollut, 2011, 215(1):127–13.Effects of Simulated Acid Rain on the Antioxidative Systemin Cinnamomum philippinenseSeedlings模拟酸雨对菲律宾樟树幼苗抗氧化系统的影响AbstractThis study examined the importance of the antioxidative defense mechanism during the applica-tion of simulated acid rain (SAR) pH 4, pH 3, pH 2, and pH 6 as control treatment in Cinnamomum philippinense seedlings. Analysis was carried out on 1, 10, and 15 days of spraying SAR. In our results, catalase (CAT), ascorbate peroxidase (APx), guaiacol peroxidase (GPOD), and glutathione reductase (GR) activity significantly were induced on 1 day of spraying SAR pH 3 and pH 2, suggesting that C. philippinense seedlings exposed to pH 3 and pH 2 acid rain for only 1 day were under oxidative stress, and antioxidant enzyme were apparently increased until 10 days of spraying SAR pH 4. However, on 10 days of spraying SAR pH 2, the accumulated intensity of acidity significantly inhibited the activi-ties of CAT and APx accompanying with increased concentrations of malonydialdehyde (MDA). On the contrary, GPOD activity and the ascorbic acid ratio were remarkably increased when spraying SAR pH 2 for 15 days. Therefore, GPOD and ascorbate contribute to the scavenging of ROS at stronger acidity stresses. However, they were not enough to avoid cellular damage, since membrane lipids were injured, and necrosis appeared.KeywordsAntioxidant enzyme .Cinnamomum philippinense . Lipid peroxidation . Simulated acid rain图1：用pH=6（对照组）的蒸馏水，pH值=4，pH=3，pH=2模拟酸雨，对菲律宾樟树幼苗叶片喷涂1天、10天 、15天后，其过氧化氢酶活性的变化。用符号（*），（**），（* * *）分别表示差异分别控制在P＜0.05，P＜0.01，P 0.001。 图2：用pH=6（对照组）的蒸馏水，pH值=4，pH=3，pH=2的模拟酸雨，对菲律宾樟树幼苗叶片喷涂1天、10天 、15天后，其抗坏血酸过氧化物酶活性的变化。统计意义如图1所示。图3：用pH=6（对照组）的蒸馏水，pH值=4，pH=3，pH=2的模拟酸雨，对菲律宾樟树幼苗叶片喷涂1天、10天、15天后，其谷胱甘肽还原酶活性的变化。统计意义如图1所示。图4：用pH=6（对照组）的蒸馏水，pH值=4，pH=3，pH=2的模拟酸雨，对菲律宾樟树幼苗叶片喷涂1天、10天、15天后，其愈创木酚过氧化物酶活性的变化。统计意义如图1所示。