补肾中药对下丘脑_垂体促性腺机能影响_沈皓.pdf

2004 1 2 1 　J Chin Integr Med, Jan 2004, Vol.2, No.1 ·53 · Original Experimental Research　 - 1 1 2 沈　皓, 蔡德培, 陈伯英 (1., 200032;2., 200032) [] 　:(gonadotropin-releasing hormone, GnRH)、 、, -。: SD (160 ～ 180 g, 1.5 ), 3 , 、 , 1 /d, 5 ml, 30 d。 、 3 GnRH Y(ne ropeptide Y, NPY); GnRH ;; GnRH NPY , 。: GnRH GnRH , 。 GnRH , GnRH , GnRH NPY , GnRH , -。GnRH NPY , GnRH , -。:GnRH 、NPY , -。 [] 　--;;;; Y;; [] 　R335.1　　[] 　A　　[]　1672-1977(2004)01-0053-05 Effects of kidney-tonifying Chinese herbal medicine on hypothalamic-pituitary gonadotrophic function 1 1 2 SHEN Hao , CAI De-Pei , CHEN Bo-Ying (1.Department of Integrated Traditional Chinese and Western Medicine, Pediatric Hospital, F dan University, Shanghai 200032, China;2.Department of Integrated Traditional Chinese and Western Medicine, Shanghai Medical College, F dan University, Shanghai 200032, China) ABSTRACT　Objective:To observe the effects of kidney-tonifying Chinese herbal medicine on the synthesis and secretion of go- nadotropin releasing hormone (GnRH)and the related ne rotransmitters and ne ropeptides, and to explore the mechanism of the reg- lative effect of Chinese herbal medicine on the hypothalamic-pit itary gonadotrophic f nction.Methods:Female Sprag e-Dawley rats d ring the period of normal adolescent initiation (160 ～ 180 g, 1.5 monthes)were randomly divided into three gro ps.The control gro p w as fed with normal saline and the tw o experimental gro ps w ere fed with Chinese herbal medicine for no rishing yin to red ce fire (Zi Yin Xie H o, ZYXH)or no rishing kidney to replenish essence (Yi Shen Tian Jing, YSTJ)respectively.The dosage was 5 ml/d for 30 days by gastric gavage.Integrated optic densities of the GnRH and ne ropeptide Y (NPY )in medial preoptic area (MPOA), arc ate n cle s (ARC)and median eminence