肿瘤坏死因子β1069位点等位基因多态性与创伤后严重脓毒血症的关系研究.pdfVIP

6 现代检验院学聋;15 2004 年 3 月 1在 19 带第 2 期 J Mod Lab Med.March 2004.Vol.19.No院 2 H中榴坏班回予日1069 位点等位基因多态性与台IJ 伤脂严敢服毒血班主的关系研究 丛 辉l ，五金湖2 ，鞠少卿1 (1. jl旨j围E在学院附属自E 院检验科，江苏两通 226001 , 2. 江苏省太仓市然…人民医院检验科，江苏太仓 215400) 摘 姿:阔的 均丁探讨江苏汉族人群中肿捕坏死隐于自(丁NFß)等位l，.阅事态性与创伤后严交.oR4串成娘 的关单。方法 采用聚合陈钝反应丽限制性片段长皮多态，陈(PCR-RFLP)方法对 58 例也，)伤后并发严重.OR J革血症病人 TNFß~ι阁的单碱占8 突变，.1-显性进行分析。在古果 i;)伤后并炭产重陈J鲁血主主病人的 TNF自祷 2 l，.网频牟较.lE.常人明.lt.升高(创伤后严重.OR~鲁鼠，班病人 68.9% ， .lE.常人 55.1% ， P 阳 Q. 015 , RR = 1.73) 。结论 TNFß 低 2 基因烦感与创伤后严重服毒血在品怠，性相关， 关键词:肿瘤坏死囚子队主主囚多~性;创伤;创伤1窗户3草除辛辛血疲 中阁分~号，Q346飞 5 R631 文献标识码，A 文意编号，1671-7414(2004)02-006-03 Study on the Relation of the Allelic Polymorphism of TNFß in 1069 Locus with Severe Sepsis in Trauma Patients CONG Hui ,W ANG lin-hu ,JU Shao-qing (1. ρepartment 01 Clinicat Laboralory , Affiliated Hospital 0/ Nantrmg Medical College ,Jiangsu Nαntong 226001 ， Chinα; 2. Department of Clinical Laboratoγy ， First Peoples H削户ital o[ 1aicang City ,Jiangsu 1aicang 215400 , China) Abstract: objcctive To approach the allelic polymorphism ofωmQr necros.í s f.a ctor ß(TNFß)and the reJation of 8. the llllel_ ic polymorphîsm of TNF自 with 时vere 时psis in trauma patients ìn the Jiangsu Han Chiese. Melhods The single base change palymorphism in TNFß gene were determined amOl1g 88 IlQrmal controls and 58 severe ttepsÎs in trauma paticnts pa- tients by using polymerase chain reaçt\on-restriction (ragment length polymorphism(PCR~RFLP). Results The gene fre- quency of ,he TNFß 怜 2 was hi日h盯 significantly in severe sepsis in 廿auma patients patÎent than ìn the normal controls(se啕 vere 削pSIS 10 trauma pat附nts 68. 9% .normal controls 55. 1% .PO. 05.RR坦1.73. Conclusion TNFß 协 2 allelic fr叫uen­ cì~s is associated with severe sepsis in trauma patients ln Jiangsu Han Chinese.