棉花TRY基因的电子克隆.pdf

新疆农业科学 2010, 47( 9) : 1709- 1713 Xinjiang Agricultural Sciences 棉花TRY 基因的电子克隆 郑 鹏, 陈全家, 高文伟, 张艳妮, 曲延英 ( 新疆农业大学农业生物技术重点实验室, 乌鲁 齐 830052) : TRIPTYCHON( TRY) RT- PCR , TRY , , TRY 142 , TRY , ORF( Open reading frame ) 270 bp, 89 TRY , , , , TRY , TRY : TRY RT - PCR : S562 S188 : A : 1001- 4330( 2010) 09 - 1709 - 05 In Silico Cloning of Triptychon in Cotton ZHENG Peng, CHEN Quan- jia, GAO Wen- w ei, ZHANG Yan- ni, Qu Yan - ying (Key Laboratory of Agricultural Biotechnology , injiang Agricultural University, Urumqi 830052, China) Abstract:Objective Cloning TRIPTYCHON ( TRY) in cotton. Method In silico cloning and RT - PCR techniques were combined to isolate a TRIPTYCHON ( TRY) gene in cotton . The character of TRY protein w ere analyzed by a series of bioinformatics softw are. Result TRIPTYCHON ( TRY) gene w as cloned in Xuzhou 142 lint- free mutants. Open reading frame ( ORF ) of TRY w as 270bp in length, encoding 89 amino acids. TRY protein have no signal peptide, and no O - glycosylation site, no transmembrane domains, and no coiled coil structure it is an unstable and hydrophobic protein , and located in the nucleus. Conclusion It is feasible to use in silico cloning techology to isolate TRY in cotton, and this research offered theoretical basis for the further research on the function of cotton TRY gene and regulatory mechanism for cotton fiber development. Key words: cotton TRIPTYCHON gene in silico cloning RT - PCR 0 引言 , [ 1] , [ 2~ 5] [ 6 ~ 8] TRY ( TRIPTYCHON) , TRY ( In silico