超声-微波协同萃取法提取黑果枸杞多糖的研究.pdfVIP

西北农业学报 　2007 ,16 ( 1) :157～158 ,175 A ct a A g ricul t u rae B oreal iocci dent al is S i nica 超声 —微波协同萃取法提取黑果枸杞多糖的研究 1 1 ,2 1 汪河滨 , 白红进 ,王金磊 ( 1. 新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室 ,新疆阿拉尔　843300 ; 2. 塔里木大学文理学院, 新疆阿拉尔　843300) 摘 　要 : 采用超声 —微波协同萃取法从黑果枸杞中提取多糖 ,并用蒽酮 —硫酸比色法测定多糖含量 。结果表 明用超声 —微波协同萃取法提取 ,测得黑果枸杞多糖含量为 10 . 89 % ,平均回收率为 100 . 07 % ,R SD = 1. 89 % (n = 3) 。该方法简单 、快速 、准确 。 关键词 : 黑果枸杞 ; 多糖 ;超声 —微波协同萃取 ;含量测定 中图分类号 :Q 658. 2 　　　　文献标识码 : A 　　　　　文章编号 (2007) 0 10 15702 Studying on UltrasonicMicrowave Synergistic Extraction Polysaccharides in L ycium r et henicum Murr WAN G Hebin1 , BA I Hongj in1 ,2 and WAN G J inlei1 ( 1. Key L aboratory of Prot ection and Utilization of Biological Resources in Tarim Ba sin , Xinj iang Production Con st ruction Corp s Alar Xinj iang 　843300 ,China ; 2 . College of Art s and Sciences , Tarim U niver sit y , Alar Xinj iang 　843300 , China) Abstract : Poly saccharide wa s ext ract ed f ro m L y ci um ret henicum Mur r an d t he co nt ent wa s det er mined . U lt ra so nicmicrowave syner gi stic ext raction t echnique app lied in poly saccharide of L y ci um re t henic um Murr were obt ained , co nt ent det er mination wit h ant hronesulp huric acid colorimet ry . Re sult s showed cont ent of poly saccharide wa s 10 . 89 % , recovery rat e i s 100 . 07 % , R SD = 1. 89 % ( n = 3) . The met ho d wa s simp le , rapid and accurat e . Key words : L y ci um ret henic um Murr ; Poly saccharide s ; U lt ra sonicmicrowave syner gi stic ext raction ; Det er mination cont ent ( )